Details:

Alternative Title:
Am J Obstet Gynecol
Personal Author:
DONG, Yafeng ; YU, Zhiyong ; SUN, Yan ; ZHOU, Hui ; STITES, Josh ; ... More +
DONG, Yafeng ; YU, Zhiyong ; SUN, Yan ; ZHOU, Hui ; STITES, Josh ; NEWELL, Katherine ; WEINER, Carl P. Less -
Description:
OBJECTIVE

The impact of chronic hypoxia on the nitric oxide synthase isoenzymes (NOSs) in specific brain structures is unknown.

STUDY DESIGN

Time-mated pregnant guinea pigs were exposed to 10.5% O2 for 14d (HPX) or room air (NMX); L-NIL (an iNOS inhibitor, 1mg/kg/day) was administered to HPX animals for 14d (L-NIL+HPX). Fetal brains were harvested at term. Multi-labeled immunofluorescence was used to generate a brain injury map. Laser capture microdissection and quantitative PCR were applied and cell injury markers, apoptosis activation, neuron loss, total NO, and the levels of individual NOSs quantified.

RESULTS

Chronic hypoxia causes selective fetal brain injury rather than globally. Injury is associated with differentially affected NO synthases in both neurons and glial cells, with iNOS up regulated at all injury sites. L-NIL attenuated the injury despite continued hypoxia.

CONCLUSIONS

These studies demonstrate chronic hypoxia selectively injures the fetal brain in part by the differential regulation of NOSs in an anatomic and cell specific manner.


Subject:
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Animals
Brain Injuries
Chronic Disease
Female
Fetal Hypoxia
Guinea Pigs
Isoenzymes
Nitric Oxide Synthase
Pregnancy
Pregnancy Complications

Source:
Am J Obstet Gynecol. 2010; 204(3):254.e16-254.e28
Pubmed ID:
21272843
Pubmed Central ID:
PMC3061276
Document Type:
Journal Article
Funding:
R03 HD062734-01A1/HD/NICHD NIH HHS/United States ; R03 HD062734/HD/NICHD NIH HHS/United States ; R01 HL049041-12/HL/NHLBI NIH HHS/United States ; R01 HL049041/HL/NHLBI NIH HHS/United States ; R01 HL049041-13/HL/NHLBI NIH HHS/United States ; ... More +
R03 HD062734-01A1/HD/NICHD NIH HHS/United States ; R03 HD062734/HD/NICHD NIH HHS/United States ; R01 HL049041-12/HL/NHLBI NIH HHS/United States ; R01 HL049041/HL/NHLBI NIH HHS/United States ; R01 HL049041-13/HL/NHLBI NIH HHS/United States ; DP00187-5/DP/NCCDPHP CDC HHS/United States ; U01 DP000187/DP/NCCDPHP CDC HHS/United States ; K12 HD065260-01/HD/NICHD NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:fd224bcefb5a17b2743db12a4ec8a13f0e0ab9eba38f0ea9e9f17632c7888d25
File Type:

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