CHRONIC FETAL HYPOXIA PRODUCES SELECTIVE BRAIN INJURY ASSOCIATED WITH ALTERED NITRIC OXIDE SYNTHASES

Details

• Alternative Title:
  Am J Obstet Gynecol
• Personal Author:
  DONG, Yafeng ; YU, Zhiyong ; SUN, Yan ; ZHOU, Hui ; STITES, Josh ; NEWELL, Katherine ; WEINER, Carl P.
• Description:
  OBJECTIVE
  
  The impact of chronic hypoxia on the nitric oxide synthase isoenzymes (NOSs) in specific brain structures is unknown.
  
  STUDY DESIGN
  
  Time-mated pregnant guinea pigs were exposed to 10.5% O2 for 14d (HPX) or room air (NMX); L-NIL (an iNOS inhibitor, 1mg/kg/day) was administered to HPX animals for 14d (L-NIL+HPX). Fetal brains were harvested at term. Multi-labeled immunofluorescence was used to generate a brain injury map. Laser capture microdissection and quantitative PCR were applied and cell injury markers, apoptosis activation, neuron loss, total NO, and the levels of individual NOSs quantified.
  
  RESULTS
  
  Chronic hypoxia causes selective fetal brain injury rather than globally. Injury is associated with differentially affected NO synthases in both neurons and glial cells, with iNOS up regulated at all injury sites. L-NIL attenuated the injury despite continued hypoxia.
  
  CONCLUSIONS
  
  These studies demonstrate chronic hypoxia selectively injures the fetal brain in part by the differential regulation of NOSs in an anatomic and cell specific manner.
  
  
• Subjects:
  Animals Brain Injuries Chronic Disease Female Fetal Hypoxia Guinea Pigs Isoenzymes Nitric Oxide Synthase Pregnancy Pregnancy Complications
• Source:
  Am J Obstet Gynecol. 2010; 204(3):254.e16-254.e28
• Pubmed ID:
  21272843
• Pubmed Central ID:
  PMC3061276
• Document Type:
  Journal Article
• Funding:
  R03 HD062734-01A1/HD/NICHD NIH HHS/United States ; R03 HD062734/HD/NICHD NIH HHS/United States ; R01 HL049041-12/HL/NHLBI NIH HHS/United States ; R01 HL049041/HL/NHLBI NIH HHS/United States ; R01 HL049041-13/HL/NHLBI NIH HHS/United States ; DP00187-5/DP/NCCDPHP CDC HHS/United States ; U01 DP000187/DP/NCCDPHP CDC HHS/United States ; K12 HD065260-01/HD/NICHD NIH HHS/United States
• Volume:
  204
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:fd224bcefb5a17b2743db12a4ec8a13f0e0ab9eba38f0ea9e9f17632c7888d25
• Download URL:
  https://stacks.cdc.gov/view/cdc/33187/cdc_33187_DS1.pdf
• File Type:
  [PDF - 2.06 MB ]