Details:

Alternative Title:
Parasit Vectors
Personal Author:
Cantillo-Barraza, Omar ; Garcés, Edilson ; Gómez-Palacio, Andrés ; Cortés, Luis A. ; Pereira, André ; ... More +
Cantillo-Barraza, Omar ; Garcés, Edilson ; Gómez-Palacio, Andrés ; Cortés, Luis A. ; Pereira, André ; Marcet, Paula L. ; Jansen, Ana M. ; Triana-Chávez, Omar Less -
Description:
Background

In Colombia, Rhodnius prolixus and Triatoma dimidiata are the main domestic triatomine species known to transmit T. cruzi. However, there are multiple reports of T. cruzi transmission involving secondary vectors. In this work, we carried out an eco-epidemiological study on Margarita Island, located in the Caribbean region of Colombia, where Chagas disease is associated with non-domiciliated vectors.

Methods

To understand the transmission dynamics of Trypanosoma cruzi in this area, we designed a comprehensive, multi-faceted study including the following: (i) entomological evaluation through a community-based insect-surveillance campaign, blood meal source determination and T. cruzi infection rate estimation in triatomine insects; (ii) serological determination of T. cruzi prevalence in children under 15 years old, as well as in domestic dogs and synanthropic mammals; (iii) evaluation of T. cruzi transmission capacity in dogs and Didelphis marsupialis, and (iv) genetic characterization of T. cruzi isolates targeting spliced-leader intergene region (SL-IR) genotypes.

Results

Out of the 124 triatomines collected, 94 % were Triatoma maculata, and 71.6 % of them were infected with T. cruzi. Blood-meal source analysis showed that T. maculata feeds on multiple hosts, including humans and domestic dogs. Serological analysis indicated 2 of 803 children were infected, representing a prevalence of 0.25 %. The prevalence in domestic dogs was 71.6 % (171/224). Domestic dogs might not be competent reservoir hosts, as inferred from negative T. cruzi xenodiagnosis and haemoculture tests. However, 61.5 % (8/13) of D. marsupialis, the most abundant synanthropic mammal captured, were T. cruzi-positive on xenodiagnosis and haemocultures.

Conclusions

This study reveals the role of peridomestic T. maculata and dogs in T. cruzi persistence in this region and presents evidence that D. marsupialis are a reservoir mediating peridomestic-zoonotic cycles. This picture reflects the complexity of the transmission dynamics of T. cruzi in an endemic area with non-domiciliated vectors where active human infection exists. There is an ongoing need to control peridomestic T. maculata populations and to implement continuous reservoir surveillance strategies with community participation.

Electronic supplementary material

The online version of this article (doi:10.1186/s13071-015-1100-2) contains supplementary material, which is available to authorized users.


Subjects:
[+]
Adolescent Animals Chagas Disease Child Child, Preschool Didelphis Disease Reservoirs Disease Transmission, Infectious Dogs Genotype Humans Insect Vectors Seroepidemiologic Studies Triatoma Trypanosoma Cruzi
Source:
Parasit Vectors. 8.
Pubmed ID:
26394766
Pubmed Central ID:
PMC4580378
Document Type:
Journal Article
Place as Subject:
Caribbean Region ; Colombia
Caribbean Region ; Colombia Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:209a9af22bd351513bf7df4a376e31742c764664a07f97c0b5f347442f422216
Download URL:
https://stacks.cdc.gov/view/cdc/34481/cdc_34481_DS1.pdf
File Type:

Supporting Files

• 	13071_2015_1100_Fig1_HTML.gif	gif
  	13071_2015_1100_Fig1_HTML.jpg	jpeg
  	13071_2015_1100_MOESM1_ESM.zip	zip
  	13071_2015_Article_1100.nxml	txt
  	license.txt	txt

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