Protein Kinase C epsilon is Important in Modulating Organic Dust-Induced Airway Inflammation

Poole, Jill A.; Romberger, Debra J.; Bauer, Chris; Gleason, Angela M.; Sisson, Joseph; Oldenburg, Peter J.; West, William W.; Wyatt, Todd A.

doi:10.3109/01902148.2012.714841

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Protein Kinase C epsilon is Important in Modulating Organic Dust-Induced Airway Inflammation

Published Date:

Aug 16 2012

Publisher's site:

http://dx.doi.org/10.3109/01902148.2012.714841 New Site Icon

Source:

Exp Lung Res. 38(8):383-395.

[PDF-1.29 MB]

Details:

Personal Authors:

Poole, Jill A. ; Romberger, Debra J. ; Bauer, Chris ; Gleason, Angela M. ; Sisson, Joseph ; ... More ▼

Keywords:

[+]

Pubmed ID:

22897707

Pubmed Central ID:

PMC4066446

Description:

Organic dust samples from swine confinement facilities elicit pro-inflammatory cytokine/chemokine release from bronchial epithelial cells and monocytes, dependent, in part, upon dust-induced activation of the protein kinase C (PKC) isoform, PKCε. PKCε is also rapidly activated in murine tracheal epithelial cells following in vivo organic dust challenges, yet the functional role of PKCε in modulating dust-induced airway inflammatory outcomes is not defined. Utilizing an established intranasal inhalation animal model, experiments investigated the biologic and physiologic responses following organic dust extract (ODE) treatments in wild-type (WT) and PKCε knock-out (KO) mice. We found that neutrophil influx increased more than twofold in PKCε KO mice following both a one-time challenge and 3 weeks of daily challenges with ODE as compared with WT mice. Lung pathology revealed increased bronchiolar and alveolar inflammation, lymphoid aggregates, and T cell influx in ODE-treated PKCε KO mice. Airway hyperresponsiveness to methacholine increased in PKCε KO + ODE to a greater magnitude than WT + ODE animals. There were no significant differences in cytokine/chemokine release elicited by ODE treatment between groups. However, ODE-induced nitric oxide (NO) production differed in that ODE exposure increased nitrate levels in WT mice but not in PKCε KO mice. Moreover, ODE failed to upregulate NO from ex vivo stimulated PKCε KO lung macrophages. Collectively, these studies demonstrate that PKCε-deficient mice were hypersensitive to organic dust exposure and suggest that PKCε is important in the normative lung inflammatory response to ODE. Dampening of ODE-induced NO may contribute to these enhanced inflammatory findings.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

ES015522-03S1/ES/NIEHS NIH HHS/United States
K08 ES015522/ES/NIEHS NIH HHS/United States
K08 ES015522-01/ES/NIEHS NIH HHS/United States
P01OH010162/OH/NIOSH CDC HHS/United States
R01 AA008769/AA/NIAAA NIH HHS/United States
R01 AA017993/AA/NIAAA NIH HHS/United States
R01 ES019325/ES/NIEHS NIH HHS/United States
R01 ES019325/ES/NIEHS NIH HHS/United States
R01AA017993/AA/NIAAA NIH HHS/United States
R01OH008539/OH/NIOSH CDC HHS/United States

Supporting Files:

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