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Nanopeptamers for the Development of Small-Analyte Lateral Flow Tests with a Positive Readout
Filetype[PDF - 1.95 MB]


Details:
  • Pubmed ID:
    23214940
  • Pubmed Central ID:
    PMC3904493
  • Funding:
    D43 TW005718/TW/FIC NIH HHS/United States
    P42 ES004699/ES/NIEHS NIH HHS/United States
    P42 ES04699/ES/NIEHS NIH HHS/United States
    TW05718/TW/FIC NIH HHS/United States
    U54 OH007550/OH/NIOSH CDC HHS/United States
  • Document Type:
  • Collection(s):
  • Description:
    There is a great demand for rapid tests that can be used on-site for the detection of small analytes, such as pesticides, persistent organic pollutants, explosives, toxins, medicinal and abused drugs, hormones, etc. Dipsticks and lateral flow devices, which are simple and provide a visual readout, may be the answer, but the available technology for these compounds requires a competitive format that loses sensitivity and produces readings inversely proportional to the analyte concentration, which is counterintuitive and may lead to potential misinterpretation of the result. In this work, protein-multipeptide constructs composed of anti-immunocomplex peptides selected from phage libraries and streptavidin/avidin as core protein were used for direct detection of small compounds in a noncompetitive two-site immunoassay format that performs with increased sensitivity and positive readout. These constructs that we termed "nanopeptamers" allow the development of rapid point-of-use tests with a positive visual end point of easy interpretation. As proof of concept, lateral flow assays for the herbicides molinate and clomazone were developed and their performance was characterized with field samples.