Nanozyme-Mediated Dual Immunoassay Integrated with Smartphone for Use in Simultaneous Detection of Pathogens

Details

• Alternative Title:
  ACS Appl Mater Interfaces
• Personal Author:
  Cheng, Nan ; Song, Yang ; Zeinhom, Mohamed M. A. ; Chang, Yu-Chung ; Sheng, Lina ; Li, Haolin ; Du, Dan ; Li, Lei ; Zhu, Mei-Jun ; Luo, Yunbo ; Xu, Wentao ; Lin, Yuehe
• Description:
  Nanozymes are an excellent class of optical reporters for the development of sensitive biosensors for widespread applications. In this study, mesoporous core-shell palladium@platinum (Pd@Pt) nanoparticles were synthesized and then applied as signal amplifier in a dual lateral flow immunoassay (LFIA) and integrated with a smartphone-based device for use in simultaneous detection of Salmonella Enteritidis and Escherichia coli O157:H7. After optimization, the limit of detections were calculated to be ∼20 cfu/mL for S. Enteritidis and ∼34 cfu/mL for E. coli O157:H7, respectively. The greatly improved sensitivity was contributed by the peroxidase-like catalytic activity of the Pd@Pt nanoparticles for signal enhancement and the parallel design of dual detection for eliminating the cross-interference. The estimated recoveries of the dual LFIA range from 91.44 to 117.00%, which indicated that the developed method is capable of detecting live bacteria in food samples. This approach provides an attractive platform for S. Enteritidis and E. coli O157:H7 detection using a smartphone-based device as the sole piece of equipment, indicating great promise for foodborne pathogen analysis or in-field food safety tracking.
• Subjects:
  Article Colony Count, Microbial E. Coli O157:H7 Escherichia Coli O157 Immunoassay Lateral Flow Immunoassay Milk Pt–Pd Nanoparticles Salmonella Enteritidis Smartphone
• Source:
  ACS Appl Mater Interfaces. 9(46):40671-40680
• Pubmed ID:
  28914522
• Pubmed Central ID:
  PMC8681872
• Document Type:
  Journal Article
• Funding:
  R01 OH011023/OH/NIOSH CDC HHSUnited States/ ; R01OH011023/ACL/ACL HHSUnited States/
• Volume:
  9
• Issue:
  46
• NIOSHTIC Number:
  nn:20065227
• Collection(s):
  CDC Public Access National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2240f591eabad95c178d0df7e5b2e7c421ba66d0236542a6a3c5f7a2e682ff964b29c7a6f9f315b38c42ad3e9ab49ef15d8fe87f8800ece518f4f28e82e575d2
• Download URL:
  https://stacks.cdc.gov/view/cdc/112805/cdc_112805_DS1.pdf
• File Type:
  [PDF - 1.65 MB ]