Electrophoretic build-up of multi nanoparticle array for a highly sensitive immunoassay

Details

• Personal Author:
  Gee SJ ; Hammock BD ; Han J-H ; Hass EA ; Kennedy IM ; Kim H-J ; Sudheendra L
• Description:
  One of the challenges in shrinking immunoassays to smaller sizes is to immobilize the biological molecules to nanometer-scaled spots.To overcome this complication, we have employed a particle- based immunoassay to create a nanostructured platform with a regular array of sensing elements. The technique makes use of an electrophoretic particle entrapment system (EPES) to immobilize nanoparticles that are coated with biological reagents into wells using a very small trapping potential. To provide useful information for controlling the trapping force and optimal design of the nanoarray, electrophoretic trapping of a nanoparticle was modeled numerically. The trapping efficiency, defined as the fraction of wells occupied by a single particle, was 91%. The performance of the array was demonstrated with a competitive immunoassay for a small molecule analyte, 3-phenoxybenzoic acid(214.2g mole-1). The limit of detection determined with a basic fluorescence microscope was 0.006 ug l-1 (30 pM); this represented a sixteen-fold improvement in sensitivity compared to a standard 96-wellplate-based ELISA; the improvement was attributed to the small size of the sample volume and the presence of light diffraction among factors unique to this structure. The EPES/nanoarray system promises to offer a new standard in applications that require portable, point-of-care and real-time monitoring with high sensitivity. [Description provided by NIOSH]
• Subjects:
  Occupational Health Particulate Matter Safety
• Keywords:
  3-phenoxybenzoic Acid (3-PBA) Author Keywords: Nanoarray Biological-function Electrophoretic Particle Entrapment System Immunoassay Models Molecular-biology Molecular-structure Nanotechnology Particulates
• ISSN:
  0956-5663
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  302-308
• Volume:
  41
• NIOSHTIC Number:
  nn:20042671
• Citation:
  Biosens Bioelectron 2013 Mar; 41:302-308
• Contact Point Address:
  Ian M. Kennedy, Department of Mechanical and Aerospace Engineering, University of California, Davis, California, CA 95616, USA
• Email:
  imkennedy@ucdavis.edu
• CAS Registry Number:
  3-Phenoxybenzoic acid (CAS RN 3739-38-6)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Biosensors and Bioelectronics
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:a1dee18e3cfb8fb5ce3a4fe61002fe94a3f0a8ee40d95cae3b2a4b5a9cfddd4ebaa963fd0a8ced67cfb1f1756885c5f5b578d071350325767cdaa9ce2dfb92f5
• Download URL:
  https://stacks.cdc.gov/view/cdc/194819/cdc_194819_DS1.pdf
• File Type:
  [PDF - 930.52 KB ]