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Structural characterization of human Uch37.
Filetype[PDF - 771.85 KB]


Details:
  • Pubmed ID:
    21953935
  • Pubmed Central ID:
    PMC3251636
  • Funding:
    DE-AC02-06CH11357/CH/OID CDC HHS/United States
    GM074901/GM/NIGMS NIH HHS/United States
    R01 LM008796/LM/NLM NIH HHS/United States
    R01 LM008796-01/LM/NLM NIH HHS/United States
    R01 LM008796-02/LM/NLM NIH HHS/United States
    R01 LM008796-03/LM/NLM NIH HHS/United States
    R01 LM008796-04/LM/NLM NIH HHS/United States
    R01-LM008796/LM/NLM NIH HHS/United States
    S10 RR13790/RR/NCRR NIH HHS/United States
    T15 LM007359/LM/NLM NIH HHS/United States
    T15 LM007359-06/LM/NLM NIH HHS/United States
    T15 LM007359-06S1/LM/NLM NIH HHS/United States
    T15 LM007359-06S2/LM/NLM NIH HHS/United States
    T15 LM007359-07/LM/NLM NIH HHS/United States
    T15 LM007359-08/LM/NLM NIH HHS/United States
    T15 LM007359-09/LM/NLM NIH HHS/United States
    T15 LM007359-09S1/LM/NLM NIH HHS/United States
    T15 LM007359-10/LM/NLM NIH HHS/United States
    T15-LM007359/LM/NLM NIH HHS/United States
    U54 GM074901/GM/NIGMS NIH HHS/United States
    U54 GM074901-01/GM/NIGMS NIH HHS/United States
    U54 GM074901-01S1/GM/NIGMS NIH HHS/United States
    U54 GM074901-02/GM/NIGMS NIH HHS/United States
    U54 GM074901-02S1/GM/NIGMS NIH HHS/United States
    U54 GM074901-03/GM/NIGMS NIH HHS/United States
    U54 GM074901-03S1/GM/NIGMS NIH HHS/United States
    U54 GM074901-04/GM/NIGMS NIH HHS/United States
    U54 GM074901-05/GM/NIGMS NIH HHS/United States
  • Document Type:
  • Collection(s):
  • Description:
    Uch37 is a de-ubiquitylating enzyme that is functionally linked with the 26S proteasome via Rpn13, and is essential for metazoan development. Here, we report the X-ray crystal structure of full-length human Uch37 at 2.95 Å resolution. Uch37's catalytic domain is similar to those of all UCH enzymes characterized to date. The C-terminal extension is elongated, predominantly helical and contains coiled coil interactions. Additionally, we provide an initial characterization of Uch37's oligomeric state and identify a systematic error in previous analyses of Uch37 activity. Taken together, these data provide a strong foundation for further analysis of Uch37's several functions.