Structural characterization of human Uch37.

Details

• Alternative Title:
  Proteins
• Personal Author:
  Burgie, E. Sethe ; Bingman, Craig A. ; Soni, Ameet B. ; Phillips, George N.
• Description:
  Uch37 is a de-ubiquitylating enzyme that is functionally linked with the 26S proteasome via Rpn13, and is essential for metazoan development. Here, we report the X-ray crystal structure of full-length human Uch37 at 2.95 Å resolution. Uch37's catalytic domain is similar to those of all UCH enzymes characterized to date. The C-terminal extension is elongated, predominantly helical and contains coiled coil interactions. Additionally, we provide an initial characterization of Uch37's oligomeric state and identify a systematic error in previous analyses of Uch37 activity. Taken together, these data provide a strong foundation for further analysis of Uch37's several functions.
• Subjects:
  Article Binding Sites Catalytic Domain Crystallography, X-Ray Deubiquitylating Enzymes Kinetics Membrane Glycoproteins Models, Molecular Proteasome Protein Assembly Protein Conformation Protein Structure, Tertiary Ubiquitin Ubiquitin C-terminal Hydrolase Ubiquitin Thiolesterase UCH-L5

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• Source:
  Proteins. 80(2):649-654
• Pubmed ID:
  21953935
• Pubmed Central ID:
  PMC3251636
• Document Type:
  Journal Article
• Funding:
  T15 LM007359-06/LM/NLM NIH HHS/United States ; R01 LM008796-04/LM/NLM NIH HHS/United States ; S10 RR013790/RR/NCRR NIH HHS/United States ; GM074901/GM/NIGMS NIH HHS/United States ; U54 GM074901-03/GM/NIGMS NIH HHS/United States ; T15 LM007359-06S2/LM/NLM NIH HHS/United States ; T15 LM007359-09/LM/NLM NIH HHS/United States ; S10 RR13790/RR/NCRR NIH HHS/United States ; T15 LM007359-09S1/LM/NLM NIH HHS/United States ; R01 LM008796-03/LM/NLM NIH HHS/United States ; T15-LM007359/LM/NLM NIH HHS/United States ; T15 LM007359/LM/NLM NIH HHS/United States ; U54 GM074901-02S1/GM/NIGMS NIH HHS/United States ; T15 LM007359-08/LM/NLM NIH HHS/United States ; T15 LM007359-06S1/LM/NLM NIH HHS/United States ; T15 LM007359-07/LM/NLM NIH HHS/United States ; U54 GM074901-05/GM/NIGMS NIH HHS/United States ; DE-AC02-06CH11357/CH/OID CDC HHS/United States ; R01 LM008796-02/LM/NLM NIH HHS/United States ; U54 GM074901-01/GM/NIGMS NIH HHS/United States ; U54 GM074901-01S1/GM/NIGMS NIH HHS/United States ; T15 LM007359-10/LM/NLM NIH HHS/United States ; U54 GM074901-02/GM/NIGMS NIH HHS/United States ; R01-LM008796/LM/NLM NIH HHS/United States ; U54 GM074901-03S1/GM/NIGMS NIH HHS/United States ; R01 LM008796-01/LM/NLM NIH HHS/United States ; R01 LM008796/LM/NLM NIH HHS/United States ; U54 GM074901/GM/NIGMS NIH HHS/United States ; U54 GM074901-04/GM/NIGMS NIH HHS/United States
• Volume:
  80
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e58ee3384f0bf9d7c4b7e931eebaea49b2e3dae73dc2fee547e59990cd67db16
• Download URL:
  https://stacks.cdc.gov/view/cdc/33291/cdc_33291_DS1.pdf
• File Type:
  [PDF - 771.85 KB ]