Human Febrile Illness Caused by Encephalomyocarditis Virus Infection, Peru

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Oberste, M. Steven ; Gotuzzo, Eduardo ; Blair, Patrick ; Nix, W. Allan ; Ksiazek, Thomas G. ; Comer, James A. ; Rollin, Pierre ; Goldsmith, Cynthia S. ; Olson, James ; Kochel, Tadeusz J.
• Description:
  Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients' clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells. Virus isolated in Vero E6 cells was identified as encephalomyocarditis virus (EMCV) by electron microscopy and by subsequent molecular diagnostic testing of samples from 2 febrile patients with nausea, headache, and dyspnea. The virus was recovered from acute-phase serum samples from both case-patients and identified with cardiovirus-specific reverse transcription-PCR and sequencing. Serum samples from case-patient 1 showed cardiovirus antibody by immunoglobulin M ELISA (acute phase <8, convalescent phase >1,024) and by neutralization assay (acute phase <10, convalescent phase >1,280). Serum samples from case-patient 2 did not contain antibodies detectable by either assay. Detection of virus in serum strongly supports a role for EMCV in human infection and febrile illness.
• Subjects:
  • Acute Disease
  • Adult
  • Animals
  • Antibodies, Viral
  • Base Sequence
  • Cardiovirus Infections
  • Cercopithecus Aethiops
  • Communicable Diseases, Emerging
  • DNA Primers
  • Encephalomyocarditis Virus
  More +
• Source:
  Emerg Infect Dis. 15(4):640-646.
• Document Type:
  Journal Article
• Place as Subject:
  Peru
• Volume:
  15
• Issue:
  4
• Download URL:
  https://stacks.cdc.gov/view/cdc/3222/cdc_3222_DS1.pdf
• File Type:
  [PDF - 356.37 KB]
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha-512:14247aa0e9414a59a36a6a0e5191beb54ff78eb0cac20923ee7cee2e9cd8ec4bdc5917f0f9b875c56894e37899e9dee1ff84be2b2cb9fc5e1b51f96b4e7f67e2