Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Oberste, M. Steven ; Gotuzzo, Eduardo ; Blair, Patrick ; Nix, W. Allan ; Ksiazek, Thomas G. ; ... More +
Oberste, M. Steven ; Gotuzzo, Eduardo ; Blair, Patrick ; Nix, W. Allan ; Ksiazek, Thomas G. ; Comer, James A. ; Rollin, Pierre ; Goldsmith, Cynthia S. ; Olson, James ; Kochel, Tadeusz J. Less -
Description:
Etiologic studies of acute febrile disease were conducted in sites across South America, including Cusco and Iquitos, Peru. Patients' clinical signs and symptoms were recorded, and acute- and convalescent-phase serum samples were obtained for serologic examination and virus isolation in Vero E6 and C6/36 cells. Virus isolated in Vero E6 cells was identified as encephalomyocarditis virus (EMCV) by electron microscopy and by subsequent molecular diagnostic testing of samples from 2 febrile patients with nausea, headache, and dyspnea. The virus was recovered from acute-phase serum samples from both case-patients and identified with cardiovirus-specific reverse transcription-PCR and sequencing. Serum samples from case-patient 1 showed cardiovirus antibody by immunoglobulin M ELISA (acute phase <8, convalescent phase >1,024) and by neutralization assay (acute phase <10, convalescent phase >1,280). Serum samples from case-patient 2 did not contain antibodies detectable by either assay. Detection of virus in serum strongly supports a role for EMCV in human infection and febrile illness.
Subject:
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Acute Disease
Adult
Animals
Antibodies, Viral
Base Sequence
Cardiovirus Infections
Cercopithecus Aethiops
Communicable Diseases, Emerging
DNA Primers
Encephalomyocarditis Virus
Female
Fever
Humans
Male
Microscopy, Electron, Transmission
Middle Aged
Phylogeny
Population Surveillance
Research
RNA, Viral
Vero Cells

Source:
Emerg Infect Dis. 15(4):640-646.
Document Type:
Journal Article
Place as Subject:
Peru
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
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urn:sha256:e8ac71a5a9c7648331f1183125383977b69281635c6974a623989aeedc0d5047
File Type:

Supporting Files

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