Imported lassa fever in Germany: molecular characterization of a new lassa virus strain.

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Günther, S. ; Emmerich, P. ; Laue, T. ; Kühle, O. ; Asper, M. ; Jung, A. ; Grewing, T. ; ter Meulen, J. ; Schmitz, H.
• Description:
  We describe the isolation and characterization of a new Lassa virus strain imported into Germany by a traveler who had visited Ghana, Côte D'Ivoire, and Burkina Faso. This strain, designated "AV," originated from a region in West Africa where Lassa fever has not been reported. Viral S RNA isolated from the patient's serum was amplified and sequenced. A long-range reverse transcription polymerase chain reaction allowed amplification of the full-length (3.4 kb) S RNA. The coding sequences of strain AV differed from those of all known Lassa prototype strains (Josiah, Nigeria, and LP) by approximately 20%, mainly at third codon positions. Phylogenetically, strain AV appears to be most closely related to strain Josiah from Sierra Leone. Lassa viruses comprise a group of genetically highly diverse strains, which has implications for vaccine development. The new method for full-length S RNA amplification may facilitate identification and molecular analysis of new arenaviruses or arenavirus strains.
• Subjects:
  Adult Animals Base Sequence Cercopithecus Aethiops Female Gene Amplification Humans Lassa Fever Lassa Virus Molecular Sequence Data Phylogeny Reverse Transcriptase Polymerase Chain Reaction Rna, Viral Travel Vero Cells

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• Source:
  Emerg Infect Dis. 6(5):466-476.
• Document Type:
  Journal Article
• Place as Subject:
  Africa ; Germany
• Location:
  Africa ; Europe
• Volume:
  6
• Issue:
  5
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:2389dd5f844e8d670237d84a4066ed4144ada5ed7f9dac629b6b048b00ad8d3f
• Download URL:
  https://stacks.cdc.gov/view/cdc/15018/cdc_15018_DS1.pdf
• File Type:
  [PDF - 246.06 KB ]