Simultaneous imaging of neural activity in three dimensions

Details

• Alternative Title:
  Front Neural Circuits
• Personal Author:
  Quirin, Sean ; Jackson, Jesse ; Peterka, Darcy S. ; Yuste, Rafael
• Description:
  We introduce a scanless optical method to image neuronal activity in three dimensions simultaneously. Using a spatial light modulator and a custom-designed phase mask, we illuminate and collect light simultaneously from different focal planes and perform calcium imaging of neuronal activity in vitro and in vivo. This method, combining structured illumination with volume projection imaging, could be used as a technological platform for brain activity mapping.
• Subjects:
  Animals Brain Activity Map Calcium Imaging Hippocampus Image Processing, Computer-Assisted Imaging, Three-Dimensional Mice Mice, Inbred C57BL Neurons Neuroscience Spatial-light-modulator Three-dimensional Imaging Volume Imaging Zebrafish

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• Source:
  Front Neural Circuits. 2014; 8.
• Pubmed ID:
  24772066
• Pubmed Central ID:
  PMC3982072
• Document Type:
  Journal Article
• Funding:
  DP1 EY024503/EY/NEI NIH HHS/United States ; DP1EY024503/DP/NCCDPHP CDC HHS/United States ; R01 EY011787/EY/NEI NIH HHS/United States ; R41MH100895/MH/NIMH NIH HHS/United States ; T32EY013933/EY/NEI NIH HHS/United States
• Volume:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7551cd1eb4f662da4a2413340f74e93619934752c4a4fc5069440c4979214af3
• Download URL:
  https://stacks.cdc.gov/view/cdc/32170/cdc_32170_DS1.pdf
• File Type:
  [PDF - 5.11 MB ]