High-throughput functional genomics using CRISPR-Cas9

Details

• Alternative Title:
  Nat Rev Genet
• Personal Author:
  Shalem, Ophir ; Sanjana, Neville E. ; Zhang, Feng
• Description:
  Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.
• Subjects:
  Animals CRISPR-Cas Systems Gene Knockout Techniques Gene Library Genetic Testing Genomics High-Throughput Nucleotide Sequencing Humans Mice Models, Genetic RNA Interference

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• Source:
  Nat Rev Genet. 2015; 16(5):299-311
• Pubmed ID:
  25854182
• Pubmed Central ID:
  PMC4503232
• Document Type:
  Journal Article
• Funding:
  DP1‑MH100706/DP/NCCDPHP CDC HHSUnited States/ ; K99 HG008171/HG/NHGRI NIH HHSUnited States/ ; K99‑HG008171/HG/NHGRI NIH HHSUnited States/ ; DP1 MH100706/MH/NIMH NIH HHSUnited States/ ; R01‑NS07312401/NS/NINDS NIH HHSUnited States/
• Volume:
  16
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:937493c62c67fe1d50ead575b4ac4ded65b37c6201da0e9b9b7e5244b9d96007
• Download URL:
  https://stacks.cdc.gov/view/cdc/32067/cdc_32067_DS1.pdf
• File Type:
  [PDF - 1.40 MB ]