Details:

Alternative Title:
Nat Rev Genet
Personal Author:
Shalem, Ophir ; Sanjana, Neville E. ; Zhang, Feng
Shalem, Ophir ; Sanjana, Neville E. ; Zhang, Feng Less -
Description:
Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unbiased, phenotypic screening. In this Review, we describe recent advances using Cas9 for genome-scale screens, including knockout approaches that inactivate genomic loci and strategies that modulate transcriptional activity. We discuss practical aspects of screen design, provide comparisons with RNA interference (RNAi) screening, and outline future applications and challenges.
Subjects:
[+]
Animals Article CRISPR-Cas Systems Gene Knockout Techniques Gene Library Genetic Testing Genomics High-Throughput Nucleotide Sequencing Humans Mice Models, Genetic RNA Interference
Source:
Nat Rev Genet. 2015; 16(5):299-311.
Pubmed ID:
25854182
Pubmed Central ID:
PMC4503232
Document Type:
Journal Article
Funding:
DP1 MH100706/MH/NIMH NIH HHS/United States ; DP1‑MH100706/DP/NCCDPHP CDC HHS/United States ; K99 HG008171/HG/NHGRI NIH HHS/United States ; K99‑HG008171/HG/NHGRI NIH HHS/United States ; R01‑NS07312401/NS/NINDS NIH HHS/United States
DP1 MH100706/MH/NIMH NIH HHS/United States ; DP1‑MH100706/DP/NCCDPHP CDC HHS/United States ; K99 HG008171/HG/NHGRI NIH HHS/United States ; K99‑HG008171/HG/NHGRI NIH HHS/United States ; R01‑NS07312401/NS/NINDS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:937493c62c67fe1d50ead575b4ac4ded65b37c6201da0e9b9b7e5244b9d96007
Download URL:
https://stacks.cdc.gov/view/cdc/32067/cdc_32067_DS1.pdf
File Type:

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