High-speed Label-free Functional Photoacoustic Microscopy of Mouse Brain in Action

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Yao, Junjie ; Wang, Lidai ; Yang, Joon-Mo ; Maslov, Konstantin I. ; Wong, Terence T. W. ; Li, Lei ; Huang, Chih-Hsien ; Zou, Jun ; Wang, Lihong V.
• Description:
  We present fast functional photoacoustic microscopy (PAM) for three-dimensional high-resolution, high-speed imaging of the mouse brain, complementary to other imaging modalities. We implemented a single-wavelength pulse-width-based method with a one-dimensional imaging rate of 100 kHz to image blood oxygenation with capillary-level resolution. We applied PAM to image the vascular morphology, blood oxygenation, blood flow and oxygen metabolism in both resting and stimulated states in the mouse brain.
• Subjects:
  Animals Blood Flow Velocity Brain Electric Stimulation Female Mice Microscopy Photoacoustic Techniques Reproducibility Of Results
• Source:
  Nat Methods. 12(5):407-410
• Pubmed ID:
  25822799
• Pubmed Central ID:
  PMC4428901
• Document Type:
  Journal Article
• Funding:
  P30 NS057105/NS/NINDS NIH HHSUnited States/ ; 1S10 RR028864/RR/NCRR NIH HHSUnited States/ ; R01 CA159959/CA/NCI NIH HHSUnited States/ ; R01 CA186567/CA/NCI NIH HHSUnited States/ ; DP1 EB016986/DP/NCCDPHP CDC HHSUnited States/ ; DP1 EB016986/EB/NIBIB NIH HHSUnited States/ ; S10 RR028864/RR/NCRR NIH HHSUnited States/
• Volume:
  12
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5f2fdeacc6bc32c25e99cfa3ee85283c9d5715bd9b11c2fcd5c2bc74f359e1ab
• Download URL:
  https://stacks.cdc.gov/view/cdc/31857/cdc_31857_DS1.pdf
• File Type:
  [PDF - 932.01 KB ]