Calibration-free in vivo transverse blood flowmetry based on cross correlation of slow-time profiles from photoacoustic microscopy

Details

• Alternative Title:
  Opt Lett
• Personal Author:
  Zhou, Yong ; Liang, Jinyang ; Maslov, Konstantin I. ; Wang, Lihong V.
• Description:
  We propose a cross-correlation-based method to measure blood-flow velocity by using photoacoustic microscopy. Unlike in previous autocorrelation-based methods, the measured flow velocity here is independent of particle size. Thus an absolute flow velocity can be obtained without calibration. We first measured the flow velocity ex vivo, using defibrinated bovine blood. Then flow velocities in vessels with different structures in a mouse ear were quantified in vivo. We further measured the flow variation in the same vessel and at a vessel bifurcation. All the experimental results indicate that our method can be used to accurately quantify blood velocity in vivo.
• Subjects:
  Animals Article Blood Flow Velocity Calibration Cattle Hemodynamics Hemorheology Mice Microscopy Photoacoustic Techniques Time Factors

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• Source:
  Opt Lett. 2013; 38(19):3882-3885.
• Pubmed ID:
  24081077
• Pubmed Central ID:
  PMC3831365
• Document Type:
  Journal Article
• Funding:
  T32 EB014855/EB/NIBIB NIH HHS/United States ; R01 CA134539/CA/NCI NIH HHS/United States ; R01 CA157277/CA/NCI NIH HHS/United States ; U54 CA136398/CA/NCI NIH HHS/United States ; R01 CA159959/CA/NCI NIH HHS/United States ; R01 EB010049/EB/NIBIB NIH HHS/United States ; DP1 EB016986/EB/NIBIB NIH HHS/United States ; R01 EB008085/EB/NIBIB NIH HHS/United States ; DP1 EB016986/DP/NCCDPHP CDC HHS/United States
• Volume:
  38
• Issue:
  19
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c966a972c841d8fdc5f8b66ae0d99850a35f3e0853330176e4d3054e07590259
• Download URL:
  https://stacks.cdc.gov/view/cdc/29777/cdc_29777_DS1.pdf
• File Type:
  [PDF - 1.11 MB ]