Wide-field two-dimensional multifocal optical-resolution photoacoustic computed microscopy

Details

• Alternative Title:
  Opt Lett
• Personal Author:
  Xia, Jun ; Li, Guo ; Wang, Lidai ; Nasiriavanaki, Mohammadreza ; Maslov, Konstantin ; Engelbach, John A. ; Garbow, Joel R. ; Wang, Lihong V.
• Description:
  Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging technique that directly images optical absorption in tissue at high spatial resolution. To date, the majority of OR-PAM systems are based on single-focused optical excitation and ultrasonic detection, limiting the wide-field imaging speed. While 1D multifocal OR-PAM (1D-MFOR-PAM) has been developed, the potential of microlens and transducer arrays has not been fully realized. Here we present the development of 2D multifocal optical-resolution photoacoustic-computed microscopy (2D-MFOR-PACM), using a 2D microlens array and a full-ring ultrasonic transducer array. The 10 mm×10 mm microlens array generates 1800 optical foci within the focal plane of the 512-element transducer array, and raster scanning the microlens array yields optical-resolution photoacoustic images. The system has improved the in-plane resolution of a full-ring transducer array from ≥100 to 29 μm and achieved an imaging time of 36 s over a 10 mm×10 mm field of view. In comparison, the 1D-MFOR-PAM would take more than 4 min to image over the same field of view. The imaging capability of the system was demonstrated on phantoms and animals both ex vivo and in vivo.
• Subjects:
  Animals Brain Female Mice Microscopy Optical Phenomena Photoacoustic Techniques Pregnancy Transducers Uterus
• Keywords:
  Maternal Uterus Microlens Array Mouse Brain Multifocal Excitation Photoacoustic Computed Microscopy Photoacoustic Computed Tomography Photoacoustic Microscopy
• Source:
  Opt Lett. 2013; 38(24):5236-5239
• Pubmed ID:
  24322226
• Pubmed Central ID:
  PMC3928040
• Document Type:
  Journal Article
• Funding:
  R01 CA134539/CA/NCI NIH HHSUnited States/ ; U54 CA136398/CA/NCI NIH HHSUnited States/ ; R01 CA159959/CA/NCI NIH HHSUnited States/ ; DP1 EB016986/EB/NIBIB NIH HHSUnited States/ ; R01 EB016963/EB/NIBIB NIH HHSUnited States/ ; R01 CA157277/CA/NCI NIH HHSUnited States/ ; R01 EB008085/EB/NIBIB NIH HHSUnited States/ ; DP1 EB016986/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  38
• Issue:
  24
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:204cf55f5b9f5a3de0bf6190ba8514250b6308c5b2929988fcf4feb4333d5528
• Download URL:
  https://stacks.cdc.gov/view/cdc/29852/cdc_29852_DS1.pdf
• File Type:
  [PDF - 1.27 MB ]