Sequencing of 16S rRNA Gene: A Rapid Tool for Identification of Bacillus anthracis

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Sacchi, Claudio T. ; Whitney, Anne M. ; Mayer, Leonard W. ; Morey, Roger ; Steigerwalt, Arnold ; Boras, Ariana ; Weyant, Robin S. ; Popovic, Tanja
• Description:
  In a bioterrorism event, a tool is needed to rapidly differentiate Bacillus anthracis from other closely related spore-forming Bacillus species. During the recent outbreak of bioterrorism-associated anthrax, we sequenced the 16S rRNA generom these species to evaluate the potential of 16S rRNA gene sequencing as a diagnostic tool. We found eight distinct 16S types among all 107 16S rRNA gene seqs fuences that differed from each other at 1 to 8 positions (0.06% to 0.5%). All 86 B. anthracis had an identical 16S gene sequence, designated type 6; 16S type 10 was seen in all B. thuringiensis strains; six other 16S types were found among the 10 B. cereus strains. This report describes the first demonstration of an exclusive association of a distinct 16S sequence with B. anthracis. Consequently, we were able to rapidly identify suspected isolates and to detect the B. anthracis 16S rRNA gene directly from culture-negative clinical specimens from seven patients with laboratory-confirmed anthrax.
• Subjects:
  16S Rrna Gene Anthrax Bacillus Anthracis Bacillus Cereus Bacillus Thuringiensis Bioterrorism Dispatch Genes, Bacterial Genetic Variation Humans Molecular Sequence Data Polymerase Chain Reaction Rna, Bacterial Rna, Ribosomal, 16S Sequence Analysis, DNA

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• Source:
  Emerg Infect Dis. 8(10):1117-1123.
• Document Type:
  Journal Article
• Place as Subject:
  Yemen
• Location:
  Yemen
• Volume:
  8
• Issue:
  10
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:b3ecc8e2de920a46741e5bd047d95ca5d5ecbbedccdf880ea710222b6adfee48
• Download URL:
  https://stacks.cdc.gov/view/cdc/3115/cdc_3115_DS1.pdf
• File Type:
  [PDF - 269.97 KB ]