Specific, Sensitive, and Quantitative Enzyme-Linked Immunosorbent Assay for Human Immunoglobulin G Antibodies to Anthrax Toxin Protective Antigen

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Quinn, Conrad P. ; Semenova, Vera A. ; Elie, Cheryl M. ; Romero-Steiner, Sandra ; Greene, Carolyn ; Li, Han ; Stamey, Karen ; Steward-Clark, Evelene ; Schmidt, Daniel S. ; Mothershed, Elizabeth ; Pruckler, Janet ; Schwartz, Stephanie ; Benson, Robert F. ; Helsel, Leta O. ; Holder, Patricia F. ; Johnson, Scott E. ; Kellum, Molly ; Messmer, Trudy ; Thacker, W. Lanier ; Besser, Lilah ; Plikaytis, Brian D. ; Taylor, Thomas H. ; Freeman, Alison E. ; Wallace, Kelly J. ; Dull, Peter ; Sejvar, Jim ; Bruce, Erica ; Moreno, Rosa ; Schuchat, Anne ; Lingappa, Jairam R. ; Martin, Sandra K. ; Walls, John ; Bronsdon, Melinda ; Carlone, George M. ; Bajani-Ari, Mary ; Ashford, David A. ; Stephens, David S. ; Perkins, Bradley A.
• Description:
  The bioterrorism-associated human anthrax epidemic in the fall of 2001 highlighted the need for a sensitive, reproducible, and specific laboratory test for the confirmatory diagnosis of human anthrax. The Centers for Disease Control and Prevention developed, optimized, and rapidly qualified an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) antibodies to Bacillus anthracis protective antigen (PA) in human serum. The qualified ELISA had a minimum detection limit of 0.06 micro g/mL, a reliable lower limit of detection of 0.09 micro g/mL, and a lower limit of quantification in undiluted serum specimens of 3.0 micro g/mL anti-PA IgG. The diagnostic sensitivity of the assay was 97.8%, and the diagnostic specificity was 97.6%. A competitive inhibition anti-PA IgG ELISA was also developed to enhance diagnostic specificity to 100%. The anti-PA ELISAs proved valuable for the confirmation of cases of cutaneous and inhalational anthrax and evaluation of patients in whom the diagnosis of anthrax was being considered.
• Subjects:
  Anthrax Antibodies, Bacterial Antigens, Bacterial Bacterial Toxins Bioterrorism Disease Outbreaks Dispatch Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin G Sensitivity And Specificity

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• Source:
  Emerg Infect Dis. 8(10):1103-1110.
• Pubmed ID:
  12396924
• Pubmed Central ID:
  PMC2730307
• Document Type:
  Journal Article
• Volume:
  8
• Issue:
  10
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:3c6eaf4b190a70aabc661392767608f53f530840ceba276a851d04f75a5c140d
• Download URL:
  https://stacks.cdc.gov/view/cdc/3113/cdc_3113_DS1.pdf
• File Type:
  [PDF - 284.73 KB ]