Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis

Details

• Alternative Title:
  J Exp Med
• Description:
  To investigate the role of Lyt-2 and Thy-1 in cytolysis, we have generated, by ethyl methanesulfonate mutagenesis and selection, variants of the cloned cytolytic T lymphocyte line L3 that specifically lack either Lyt-2 or Thy-1. An analysis of these variants indicates that neither Lyt-2 nor Lyt-3 is responsible for the lethal hit, but suggests that Lyt-2 and/or Lyt-3 are required for an antigen receptor functional in cytolysis. The data also suggest that the expression of Lyt-3 on the cell surface is not independent of the expression of Lyt-2. Finally the data indicate the Thy-1 plays no role in cytolysis.
• Subjects:
  Animals Antibodies Antibodies, Monoclonal Antigens, Surface Binding, Competitive Cells, Cultured Clone Cells Concanavalin A Cytotoxicity, Immunologic Ethyl Methanesulfonate Female Genetic Variation Isoantigens Mice Mice, Inbred C57BL Mice, Inbred DBA Phenotype Rats Rats, Inbred Lew Receptors, Antigen, T-Cell

  More +
• Source:
  J Exp Med. 153(3):595-604.
• Pubmed ID:
  6788878
• Pubmed Central ID:
  PMC2186095
• Document Type:
  Journal Article
• Volume:
  153
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:1fe312fc4b5af323f874dd18d31464b0058a22af48691ea57b49cf59a899dae2
• Download URL:
  https://stacks.cdc.gov/view/cdc/30799/cdc_30799_DS1.pdf
• File Type:
  [PDF - 752.12 KB ]