Details:

Alternative Title:
Nat Neurosci
Personal Author:
Chuong, Amy S ; Miri, Mitra L ; Busskamp, Volker ; Matthews, Gillian A C ; Acker, Leah C ; ... More +
Chuong, Amy S ; Miri, Mitra L ; Busskamp, Volker ; Matthews, Gillian A C ; Acker, Leah C ; Sørensen, Andreas T ; Young, Andrew ; Klapoetke, Nathan C ; Henninger, Mike A ; Kodandaramaiah, Suhasa B ; Ogawa, Masaaki ; Ramanlal, Shreshtha B ; Bandler, Rachel C ; Allen, Brian D ; Forest, Craig R ; Chow, Brian Y ; Han, Xue ; Lin, Yingxi ; Tye, Kay M ; Roska, Botond ; Cardin, Jessica A ; Boyden, Edward S ; Less -
Description:
Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.
Subject:
[+]
Animals
Article
Brain Chemistry
Halobacterium Salinarum
Halorhodopsins
Mice
Molecular Sequence Data
Neural Inhibition
Neurons
Optogenetics
Retina

Source:
Nat Neurosci. 17(8):1123-1129.
Pubmed ID:
24997763
Pubmed Central ID:
PMC4184214
Document Type:
Journal Article ;
Funding:
1DP2NS082126/DP/NCCDPHP CDC HHS/United States ; 1DP2OD002002/OD/NIH HHS/United States ; 1R01DA029639/DA/NIDA NIH HHS/United States ; 1R01NS067199/NS/NINDS NIH HHS/United States ; 1R01NS075421/NS/NINDS NIH HHS/United States ; ... More +
1DP2NS082126/DP/NCCDPHP CDC HHS/United States ; 1DP2OD002002/OD/NIH HHS/United States ; 1R01DA029639/DA/NIDA NIH HHS/United States ; 1R01NS067199/NS/NINDS NIH HHS/United States ; 1R01NS075421/NS/NINDS NIH HHS/United States ; 1R01NS081716/NS/NINDS NIH HHS/United States ; 1R01NS087950/NS/NINDS NIH HHS/United States ; 1R21NS078660/NS/NINDS NIH HHS/United States ; 1RC1MH088182/MH/NIMH NIH HHS/United States ; 5R00MH085944/MH/NIMH NIH HHS/United States ; DP2 DK102256/DK/NIDDK NIH HHS/United States ; DP2 NS082126/NS/NINDS NIH HHS/United States ; DP2-OD-017366-01/OD/NIH HHS/United States ; P30 ES002109/ES/NIEHS NIH HHS/United States ; R00 EY018407/EY/NEI NIH HHS/United States ; R01 EY022951/EY/NEI NIH HHS/United States ; R01 EY022951/EY/NEI NIH HHS/United States ; R01 MH091220-01/MH/NIMH NIH HHS/United States ; R01 MH102365/MH/NIMH NIH HHS/United States ; R01 MH102365/MH/NIMH NIH HHS/United States ; R01 MH102441/MH/NIMH NIH HHS/United States ; R01-MH102441-01/MH/NIMH NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:5d45cba6a73e3a66ce64ef3020739e6c14f6b4083f4e83f8dcd4131f0fa234c2
File Type:

Supporting Files

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  	NIHMS621295-supplement-01.pdf	pdf
  	NIHMS621295-supplement-Supplementary_Methods.pdf	pdf
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