Noninvasive optical inhibition with a red-shifted microbial rhodopsin

Details

• Alternative Title:
  Nat Neurosci
• Personal Author:
  Chuong, Amy S ; Miri, Mitra L ; Busskamp, Volker ; Matthews, Gillian A C ; Acker, Leah C ; Sørensen, Andreas T ; Young, Andrew ; Klapoetke, Nathan C ; Henninger, Mike A ; Kodandaramaiah, Suhasa B ; Ogawa, Masaaki ; Ramanlal, Shreshtha B ; Bandler, Rachel C ; Allen, Brian D ; Forest, Craig R ; Chow, Brian Y ; Han, Xue ; Lin, Yingxi ; Tye, Kay M ; Roska, Botond ; Cardin, Jessica A ; Boyden, Edward S
• Description:
  Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light-induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.
• Subjects:
  Animals Brain Chemistry Halobacterium Salinarum Halorhodopsins Mice Molecular Sequence Data Neural Inhibition Neurons Optogenetics Retina
• Source:
  Nat Neurosci. 17(8):1123-1129
• Pubmed ID:
  24997763
• Pubmed Central ID:
  PMC4184214
• Document Type:
  Journal Article
• Funding:
  1R01NS081716/NS/NINDS NIH HHSUnited States/ ; R00 EY018407/EY/NEI NIH HHSUnited States/ ; RC1 MH088182/MH/NIMH NIH HHSUnited States/ ; 1R01NS075421/NS/NINDS NIH HHSUnited States/ ; R01 EY023173/EY/NEI NIH HHSUnited States/ ; R00 MH085944/MH/NIMH NIH HHSUnited States/ ; 5R00MH085944/MH/NIMH NIH HHSUnited States/ ; R01 MH102365/MH/NIMH NIH HHSUnited States/ ; R01 NS075421/NS/NINDS NIH HHSUnited States/ ; R01 NS067199/NS/NINDS NIH HHSUnited States/ ; R01 DA029639/DA/NIDA NIH HHSUnited States/ ; R01 MH091220-01/MH/NIMH NIH HHSUnited States/ ; 1R01NS087950/NS/NINDS NIH HHSUnited States/ ; R01 EY022951/EY/NEI NIH HHSUnited States/ ; DP2 OD002002/OD/NIH HHSUnited States/ ; 1DP2NS082126/DP/NCCDPHP CDC HHSUnited States/ ; P30 ES002109/ES/NIEHS NIH HHSUnited States/ ; R01 MH091220/MH/NIMH NIH HHSUnited States/ ; DP2 NS082126/NS/NINDS NIH HHSUnited States/ ; R01 NS087950/NS/NINDS NIH HHSUnited States/ ; DP2 DK102256/DK/NIDDK NIH HHSUnited States/ ; R01 MH102441/MH/NIMH NIH HHSUnited States/ ; 1RC1MH088182/MH/NIMH NIH HHSUnited States/ ; 1R01DA029639/DA/NIDA NIH HHSUnited States/ ; DP2-OD-017366-01/OD/NIH HHSUnited States/ ; R01-MH102441-01/MH/NIMH NIH HHSUnited States/ ; R01 NS081716/NS/NINDS NIH HHSUnited States/ ; 1DP2OD002002/OD/NIH HHSUnited States/ ; 1R01NS067199/NS/NINDS NIH HHSUnited States/ ; R21 NS078660/NS/NINDS NIH HHSUnited States/ ; 1R21NS078660/NS/NINDS NIH HHSUnited States/
• Volume:
  17
• Issue:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5d45cba6a73e3a66ce64ef3020739e6c14f6b4083f4e83f8dcd4131f0fa234c2
• Download URL:
  https://stacks.cdc.gov/view/cdc/30136/cdc_30136_DS1.pdf
• File Type:
  [PDF - 1.73 MB ]