Optical-Resolution Photoacoustic Microscopy for Volumetric and Spectral Analysis of Histological and Immunochemical Samples**

Details

• Alternative Title:
  Angew Chem Int Ed Engl
• Personal Author:
  Zhang, Yu Shrike ; Yao, Junjie ; Zhang, Chi ; Li, Lei ; Wang, Lihong V. ; Xia, Younan
• Description:
  Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.
• Subjects:
  Article Biomedical Imaging Cell Biology Histochemistry Microscopy Photoacoustic Microscopy Photoacoustic Techniques Spectrophotometry, Ultraviolet Tissue Engineering
• Source:
  Angew Chem Int Ed Engl. 53(31):8099-8103
• Pubmed ID:
  24961608
• Pubmed Central ID:
  PMC4116434
• Document Type:
  Journal Article
• Funding:
  P30 NS057105/NS/NINDS NIH HHSUnited States/ ; R01 CA134539/CA/NCI NIH HHSUnited States/ ; DP1 OD000798/OD/NIH HHSUnited States/ ; R01 CA186567/CA/NCI NIH HHSUnited States/ ; DP1 EB016986/EB/NIBIB NIH HHSUnited States/ ; DP1 EB016986/DP/NCCDPHP CDC HHSUnited States/ ; R01 EB016963/EB/NIBIB NIH HHSUnited States/ ; R01 CA157277/CA/NCI NIH HHSUnited States/ ; R01 CA159959/CA/NCI NIH HHSUnited States/ ; R01 EB010049/EB/NIBIB NIH HHSUnited States/
• Volume:
  53
• Issue:
  31
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5dba4d044fda57551d269193fb74a176ea1e5f9c54b3cca100cec1970530352d
• Download URL:
  https://stacks.cdc.gov/view/cdc/30055/cdc_30055_DS1.pdf
• File Type:
  [PDF - 1.86 MB ]