Optical-Resolution Photoacoustic Microscopy for Volumetric and Spectral Analysis of Histological and Immunochemical Samples**

Zhang, Yu Shrike; Yao, Junjie; Zhang, Chi; Li, Lei; Wang, Lihong V.; Xia, Younan

doi:10.1002/anie.201403812

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Optical-Resolution Photoacoustic Microscopy for Volumetric and Spectral Analysis of Histological and Immunochemical Samples**

Published Date:

Jun 24 2014

Publisher's site:

http://dx.doi.org/10.1002/anie.201403812 New Site Icon

Source:

Angew Chem Int Ed Engl. 53(31):8099-8103.

[PDF-1.86 MB]

Details:

Personal Authors:

Zhang, Yu Shrike ; Yao, Junjie ; Zhang, Chi ; Li, Lei ; Wang, Lihong V. ; ... More ▼

Keywords:

[+]

Pubmed ID:

24961608

Pubmed Central ID:

PMC4116434

Description:

Optical-resolution photoacoustic microscopy (OR-PAM) is an imaging modality with superb penetration depth and excellent absorption contrast. Here we demonstrate, for the first time, that this technique can advance quantitative analysis of conventional chromogenic histochemistry. Because OR-PAM can quantify the absorption contrast at different wavelengths, it is feasible to spectrally resolve the specific biomolecules involved in a staining color. Furthermore, the tomographic capability of OR-PAM allows for noninvasive volumetric imaging of a thick sample without microtoming it. By immunostaining the sample with different chromogenic agents, we further demonstrated the ability of OR-PAM to resolve different types of cells in a coculture sample with imaging depths up to 1 mm. Taken together, the integration of OR-PAM with (immuno)histochemistry offers a simple and versatile technique with broad applications in cell biology, pathology, tissue engineering, and related biomedical studies.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

Supporting Files:

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