Three-dimensional arbitrary trajectory scanning photoacoustic microscopy

Yeh, Chenghung; Soetikno, Brian; Hu, Song; Maslov, Konstantin I.; Wang, Lihong V.

doi:10.1002/jbio.201400055

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i

Three-dimensional arbitrary trajectory scanning photoacoustic microscopy

4 2015
By Yeh, Chenghung ; Soetikno, Brian ; Hu, Song ; ...
http://dx.doi.org/10.1002/jbio.201400055
Source: J Biophotonics. 2014; 8(4):303-308

[PDF-842.32 KB]

English

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Details:

Alternative Title:

J Biophotonics
Personal Author:

Yeh, Chenghung ; Soetikno, Brian ; Hu, Song ; Maslov, Konstantin I. ; Wang, Lihong V.

Yeh, Chenghung ; Soetikno, Brian ; Hu, Song ; Maslov, Konstantin I. ; Wang, Lihong V. Less -
Description:

We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2 ) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method.
Subjects:

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Animals Ear Hair Hemoglobins Humans Imaging, Three-Dimensional Mice Microscopy Oxygen Photoacoustic Techniques
Keywords:

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3-D Arbitrary Trajectory Scanning Hemoglobin Oxygen Saturation Metabolic Rate Of Oxygen Optical-resolution Photoacoustic Microscopy Raster Scanning
Source:

J Biophotonics. 2014; 8(4):303-308
Pubmed ID:

25077689
Pubmed Central ID:

PMC4312269
Document Type:

Journal Article
Funding:

R01 EB016963/EB/NIBIB NIH HHSUnited States/ ; R01 CA159959/CA/NCI NIH HHSUnited States/ ; R01 CA186567/CA/NCI NIH HHSUnited States/ ; DP1 EB016986/EB/NIBIB NIH HHSUnited States/ ; DP1 EB016986/DP/NCCDPHP CDC HHSUnited States/

R01 EB016963/EB/NIBIB NIH HHSUnited States/ ; R01 CA159959/CA/NCI NIH HHSUnited States/ ; R01 CA186567/CA/NCI NIH HHSUnited States/ ; DP1 EB016986/EB/NIBIB NIH HHSUnited States/ ; DP1 EB016986/DP/NCCDPHP CDC HHSUnited States/ Less -
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:e64a763898bb7303fcf5cbaf0341897923e49566b13ea24b18e8d81cd459480e
Download URL:

https://stacks.cdc.gov/view/cdc/31307/cdc_31307_DS1.pdf
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