Three-dimensional arbitrary trajectory scanning photoacoustic microscopy

Details

• Alternative Title:
  J Biophotonics
• Personal Author:
  Yeh, Chenghung ; Soetikno, Brian ; Hu, Song ; Maslov, Konstantin I. ; Wang, Lihong V.
• Description:
  We have enhanced photoacoustic microscopy with three-dimensional arbitrary trajectory (3-DAT) scanning, which can rapidly image selected vessels over a large field of view (FOV) and maintain a high signal-to-noise ratio (SNR) despite the depth variation of the vessels. We showed that hemoglobin oxygen saturation (sO2 ) and blood flow can be measured simultaneously in a mouse ear in vivo at a frame rate 67 times greater than that of a traditional two-dimensional raster scan. We also observed sO2 dynamics in response to switching from systemic hypoxia to hyperoxia. 3-DAT-scanning photoacoustic microscopy. Schematic diagram of the 3D scanning stage and method.
• Subjects:
  Animals Ear Hair Hemoglobins Humans Imaging, Three-Dimensional Mice Microscopy Oxygen Photoacoustic Techniques
• Keywords:
  3-D Arbitrary Trajectory Scanning Hemoglobin Oxygen Saturation Metabolic Rate Of Oxygen Optical-resolution Photoacoustic Microscopy Raster Scanning
• Source:
  J Biophotonics. 2014; 8(4):303-308
• Pubmed ID:
  25077689
• Pubmed Central ID:
  PMC4312269
• Document Type:
  Journal Article
• Funding:
  R01 EB016963/EB/NIBIB NIH HHSUnited States/ ; R01 CA159959/CA/NCI NIH HHSUnited States/ ; R01 CA186567/CA/NCI NIH HHSUnited States/ ; DP1 EB016986/EB/NIBIB NIH HHSUnited States/ ; DP1 EB016986/DP/NCCDPHP CDC HHSUnited States/
• Volume:
  8
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e64a763898bb7303fcf5cbaf0341897923e49566b13ea24b18e8d81cd459480e
• Download URL:
  https://stacks.cdc.gov/view/cdc/31307/cdc_31307_DS1.pdf
• File Type:
  [PDF - 842.32 KB ]