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Characterization of cardiolipins and their oxidation products by LC-MS analysis
Filetype[PDF - 1.66 MB]


Details:
  • Pubmed ID:
    24333544
  • Pubmed Central ID:
    PMC4025908
  • Funding:
    ES020693/ES/NIEHS NIH HHS/United States
    ES021068/ES/NIEHS NIH HHS/United States
    NS061817/NS/NINDS NIH HHS/United States
    NS076511/NS/NINDS NIH HHS/United States
    OH008282/OH/NIOSH CDC HHS/United States
    R01 ES020693/ES/NIEHS NIH HHS/United States
    R01 NS061817/NS/NINDS NIH HHS/United States
    R01 NS076511/NS/NINDS NIH HHS/United States
    R21 ES021068/ES/NIEHS NIH HHS/United States
    U19 AI068021/AI/NIAID NIH HHS/United States
    U19AI068021/AI/NIAID NIH HHS/United States
  • Document Type:
  • Collection(s):
  • Description:
    Cardiolipins, a class of mitochondria-specific lipid molecules, is one of the most unusual and ancient phospholipids found in essentially all living species. Typical of mammalian cells is the presence of vulnerable to oxidation polyunsaturated fatty acid resides in CL molecules. The overall role and involvement of cardiolipin oxidation (CLox) products in major intracellular signaling as well as extracellular inflammatory and immune responses have been established. However, identification of individual peroxidized molecular species in the context of their ability to induce specific biological responses has not been yet achieved. This is due, at least in part, to technological difficulties in detection, identification, structural characterization and quantitation of CLox associated with their very low abundance and exquisite diversification. This dictates the need for the development of new methodologies for reliable, sensitive and selective analysis of both CLox. LC-MS-based oxidative lipidomics with high mass accuracy instrumentation as well as new software packages are promising in achieving the goals of expedited and reliable analysis of cardiolipin oxygenated species in biosamples.