Details:

Alternative Title:
Mol Nutr Food Res
Personal Author:
Tyurina, Yulia Y. ; Winnica, Daniel E. ; Kapralova, Valentina I. ; Kapralov, Alexandr A. ; Tyurin, Vladimir A. ; ... More +
Tyurina, Yulia Y. ; Winnica, Daniel E. ; Kapralova, Valentina I. ; Kapralov, Alexandr A. ; Tyurin, Vladimir A. ; Kagan, Valerian E. Less -
Description:
Scope

Rotenone is a toxicant believed to contribute to the development of Parkinson's disease.

Methods and results

Using human peripheral blood lymphocytes we demonstrated that exposure to rotenone resulted in disruption of electron transport accompanied by the production of reactive oxygen species, development of apoptosis and elevation of peroxidase activity of mitochondria. Employing LC/MS based lipidomics/oxidative lipidomics we characterized molecular species of cardiolipin (CL) and its oxidation/hydrolysis products formed early in apoptosis and associated with the rotenone-induced mitochondrial dysfunction.

Conclusions

The major oxidized CL species - tetra-linoleoyl-CL – underwent oxidation to yield epoxy-C18:2 and dihydroxy-C18:2 derivatives predominantly localized in sn-1 and sn-2 positions, respectively. In addition, accumulation of mono-lyso-CL species and oxygenated free C18:2 were detected in rotenone-treated lymphocytes. These oxidation/hydrolysis products may be useful for the development of new biomarkers of mitochondrial dysfunction.


Subjects:
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Apoptosis Article Cardiolipin Peroxidation Cardiolipins Cells, Cultured Chromatography, Liquid Humans Lymphocytes Lysophospholipids Mass Spectrometry Mitochondria Mitochondrial Dysfunction Oxidation-Reduction Parkinson's Disease Biomarkers Peroxidases Reactive Oxygen Species Rotenone
Source:
Mol Nutr Food Res. 57(8):1410-1422.
Pubmed ID:
23650208
Pubmed Central ID:
PMC3810210
Document Type:
Journal Article
Funding:
ES020693/ES/NIEHS NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; R01 ES020693/ES/NIEHS NIH HHS/United States ; R01 HL070755/HL/NHLBI NIH HHS/United States ; ... More +
ES020693/ES/NIEHS NIH HHS/United States ; HL70755/HL/NHLBI NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; R01 ES020693/ES/NIEHS NIH HHS/United States ; R01 HL070755/HL/NHLBI NIH HHS/United States ; U19 AI068021/AI/NIAID NIH HHS/United States ; U19AIO68021/PHS HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:c51f75fe711462aa2d523a9f565c68daff4d9e8dbdde2bdc09032179adf6f111
Download URL:
https://stacks.cdc.gov/view/cdc/29765/cdc_29765_DS1.pdf
File Type:

Supporting Files

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