LC/MS characterization of rotenone induced cardiolipin oxidation in human lymphocytes: Implications for mitochondrial dysfunction associated with Parkinson's disease

Tyurina, Yulia Y.; Winnica, Daniel E.; Kapralova, Valentina I.; Kapralov, Alexandr A.; Tyurin, Vladimir A.; Kagan, Valerian E.

doi:10.1002/mnfr.201200801

Advanced Search

Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

Advanced Search
Custom Query

All these words:

For very narrow results

This exact word or phrase:

When looking for a specific result

Any of these words:

Best used for discovery & interchangable words

None of these words:

Recommended to be used in conjunction with other fields

Language:

Dates

Publication Date Range:

to

Document Data

Title:

Document Type:

Library

Collection:

Series:

People

Author:

Clear All

Query Builder

Query box

Clear All

For additional assistance using the Custom Query please check out our Help Page

i

LC/MS characterization of rotenone induced cardiolipin oxidation in human lymphocytes: Implications for mitochondrial dysfunction associated with Parkinson's disease

8 2013
By Tyurina, Yulia Y. ; Winnica, Daniel E. ; Kapralova, Valentina I. ; ...
http://dx.doi.org/10.1002/mnfr.201200801
Source: Mol Nutr Food Res. 57(8):1410-1422

[PDF-947.22 KB]

English

Details Supporting Files You May Also Like

Details:

Alternative Title:

Mol Nutr Food Res
Personal Author:

Tyurina, Yulia Y. ; Winnica, Daniel E. ; Kapralova, Valentina I. ; Kapralov, Alexandr A. ; Tyurin, Vladimir A. ; ... More +

Tyurina, Yulia Y. ; Winnica, Daniel E. ; Kapralova, Valentina I. ; Kapralov, Alexandr A. ; Tyurin, Vladimir A. ; Kagan, Valerian E. Less -
Description:

Scope

Rotenone is a toxicant believed to contribute to the development of Parkinson's disease.

Methods and results

Using human peripheral blood lymphocytes we demonstrated that exposure to rotenone resulted in disruption of electron transport accompanied by the production of reactive oxygen species, development of apoptosis and elevation of peroxidase activity of mitochondria. Employing LC/MS based lipidomics/oxidative lipidomics we characterized molecular species of cardiolipin (CL) and its oxidation/hydrolysis products formed early in apoptosis and associated with the rotenone-induced mitochondrial dysfunction.

Conclusions

The major oxidized CL species - tetra-linoleoyl-CL – underwent oxidation to yield epoxy-C18:2 and dihydroxy-C18:2 derivatives predominantly localized in sn-1 and sn-2 positions, respectively. In addition, accumulation of mono-lyso-CL species and oxygenated free C18:2 were detected in rotenone-treated lymphocytes. These oxidation/hydrolysis products may be useful for the development of new biomarkers of mitochondrial dysfunction.
Subjects:

[+]

Apoptosis Cardiolipins Cells, Cultured Chromatography, Liquid Humans Lymphocytes Lysophospholipids Mass Spectrometry Mitochondria Oxidation-Reduction Peroxidases Reactive Oxygen Species Rotenone
Keywords:

[+]

Apoptosis Cardiolipin Peroxidation Lymphocytes Mitochondrial Dysfunction Parkinson's Disease Biomarkers
Source:

Mol Nutr Food Res. 57(8):1410-1422
Pubmed ID:

23650208
Pubmed Central ID:

PMC3810210
Document Type:

Journal Article
Funding:

R01 HL070755/HL/NHLBI NIH HHSUnited States/ ; R01 ES020693/ES/NIEHS NIH HHSUnited States/ ; R01 OH008282/OH/NIOSH CDC HHSUnited States/ ; U19 AI068021/AI/NIAID NIH HHSUnited States/ ; U19AIO68021/PHS HHSUnited States/ ; ... More +

R01 HL070755/HL/NHLBI NIH HHSUnited States/ ; R01 ES020693/ES/NIEHS NIH HHSUnited States/ ; R01 OH008282/OH/NIOSH CDC HHSUnited States/ ; U19 AI068021/AI/NIAID NIH HHSUnited States/ ; U19AIO68021/PHS HHSUnited States/ ; ES020693/ES/NIEHS NIH HHSUnited States/ ; OH008282/OH/NIOSH CDC HHSUnited States/ ; HL70755/HL/NHLBI NIH HHSUnited States/ Less -
Volume:

57
Issue:

8
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:c51f75fe711462aa2d523a9f565c68daff4d9e8dbdde2bdc09032179adf6f111
Download URL:

https://stacks.cdc.gov/view/cdc/29765/cdc_29765_DS1.pdf
File Type:

	nihms-515225-f0005.gif	gif
	nihms-515225-f0005.jpg	jpeg
	nihms-515225-f0006.gif	gif
	nihms-515225-f0006.jpg	jpeg
	nihms-515225-f0007.gif	gif
	nihms-515225-f0007.jpg	jpeg
	nihms-515225-f0008.gif	gif
	nihms-515225-f0008.jpg	jpeg
	nihms-515225-f0009.gif	gif
	nihms-515225-f0009.jpg	jpeg
	nihms-515225-f0001.gif	gif
	nihms-515225.nxml	xml
	NIHMS515225-supplement-Supplementary_Figures.pptx	pptx
	nihms-515225-f0001.jpg	jpeg
	nihms-515225-f0002.gif	gif
	nihms-515225-f0002.jpg	jpeg
	nihms-515225-f0003.gif	gif
	nihms-515225-f0003.jpg	jpeg
	nihms-515225-f0004.gif	gif
	nihms-515225-f0004.jpg	jpeg

More +