Efficient delivery of lentiviral vectors into resting human CD4 T cells

Details

• Alternative Title:
  Gene Ther
• Personal Author:
  Geng, Xin ; Doitsh, Gilad ; Yang, Zhiyuan ; Galloway, Nicole LK ; Greene, Warner C.
• Description:
  Resting human CD4 T cells are highly resistant to transfection or infection with lentiviral vectors derived from the human immunodeficiency virus. We now describe a flexible and efficient approach involving virus-like particles containing simian immunodeficiency virus lentiviral gene product protein X and pseudotyping with CXCR4-tropic HIV Env. This method permits effective genetic manipulation of these cells while preserving their naturally quiescent state. This technology can also be extended to primary lymphoid cultures where authentic cellular composition and functional relationships are preserved.
• Subjects:
  Animals CD4-Positive T-Lymphocytes Env Gene Products, Human Immunodeficiency Virus Genetic Therapy Genetic Vectors Gene Transfer Techniques HIV-1 Humans Lentivirus Receptors, CXCR4 Simian Immunodeficiency Virus Viral Proteins

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• Keywords:
  AIDS CD4 T Cells HIV ShRNA Vpx
• Source:
  Gene Ther. 21(4):444-449
• Pubmed ID:
  24572792
• Pubmed Central ID:
  PMC4006095
• Document Type:
  Journal Article
• Funding:
  NIH/NIAID R21 AI102782/PHS HHSUnited States/ ; T32 AI060537/AI/NIAID NIH HHSUnited States/ ; P30 AI027763/AI/NIAID NIH HHSUnited States/ ; R21 AI102782/AI/NIAID NIH HHSUnited States/ ; 1DP11036502/DP/NCCDPHP CDC HHSUnited States/ ; U19 AI096113/AI/NIAID NIH HHSUnited States/ ; NIH/NIAID U19 AI096113/PHS HHSUnited States/ ; DP1 DA036502/DA/NIDA NIH HHSUnited States/
• Volume:
  21
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:5b9ace2dc61982b2f8529f04e962492e9ab0a8614f47a27b3def7131eb0ed5d4
• Download URL:
  https://stacks.cdc.gov/view/cdc/29943/cdc_29943_DS1.pdf
• File Type:
  [PDF - 552.74 KB ]