Improved Spatial Resolution of MALDI Imaging of Lipids in the Brain by Alkylated Derivatives of 2,5-Dihydroxybenzoic Acid

Details

• Alternative Title:
  Rapid Commun Mass Spectrom
• Personal Author:
  Stoyanovsky, D.A. ; Sparvero, L.J. ; Amoscato, A.A. ; He, R.R. ; Watkins, S. ; Pitt, B.R. ; Bayir, H. ; Kagan, V.E.
• Description:
  Matrix-assisted laser desorption/ionization (MALDI) is one of the major techniques for mass spectrometry imaging (MSI) of biological systems along with secondary-ion mass spectrometry (SIMS) and desorption electrospray mass spectrometry (DESI). The inherent variability of MALDI-MSI signals within intact tissues is related to the heterogeneity of both the sample surface and the matrix crystallization. To circumvent some of these limitations of MALDI-MSI, we have developed improved matrices for lipid analysis based on structural modification of the commonly used matrix 2,5-dihydroxybenzoic acid (DHB).|We have synthesized DHB containing -C6H13 and -C12H25 alkyl chains and applied these matrices to rat brain using a capillary sprayer. We utilized a Bruker Ultraflex II MALDI-TOF/TOF mass spectrometer to analyze lipid extracts and tissue sections, and examined these sections with polarized light microscopy and differential interference contrast microscopy.|O-alkylation of DHB yields matrices, which, when applied to brain sections, follow a trend of phase transition from crystals to an oily layer in the sequence DHB → DHB-C6H13 → DHB-C12H25 . MALDI-MSI images acquired with DHB-C12H25 exhibited a considerably higher density of lipids than DHB.|Comparative experiments with DHB and DHB-C12H25 are presented, which indicate that the latter matrix affords higher lateral resolution than the former.
• Subjects:
  Animals Article Brain Chemistry Gentisates Histocytochemistry Lipids Male Molecular Imaging Rats Rats, Sprague-Dawley Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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• Source:
  Rapid Commun Mass Spectrom. 28(5):403-412.
• Pubmed ID:
  24497278
• Pubmed Central ID:
  PMC3973445
• Document Type:
  Journal Article
• Funding:
  ES020693/ES/NIEHS NIH HHS/United States ; ES021068/ES/NIEHS NIH HHS/United States ; NS061817/NS/NINDS NIH HHS/United States ; NS076511/NS/NINDS NIH HHS/United States ; OH008282/OH/NIOSH CDC HHS/United States ; P30CA047904/CA/NCI NIH HHS/United States ; R01 NS061817/NS/NINDS NIH HHS/United States ; R01 NS076511/NS/NINDS NIH HHS/United States ; U19 AI068021/AI/NIAID NIH HHS/United States ; U19AI068021/AI/NIAID NIH HHS/United States ; U54 GM103529 08/GM/NIGMS NIH HHS/United States ; UL1 TR000005/TR/NCATS NIH HHS/United States
• Volume:
  28
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:e6ea5d2bcc4e78ce2e717f822ef51d24559276cc713cad13db83d486b718e2bd
• Download URL:
  https://stacks.cdc.gov/view/cdc/29905/cdc_29905_DS1.pdf
• File Type:
  [PDF - 2.91 MB ]