Imaging Mass Spectrometry Reveals Loss of Polyunsaturated Cardiolipins in the Cortical Contusion, Hippocampus and Thalamus after Traumatic Brain Injury

Details

• Alternative Title:
  J Neurochem
• Personal Author:
  Sparvero, L. J. ; Amoscato, A. A. ; Fink, A. B. ; Anthonymuthu, T. ; New, L.E. ; Kochanek, P.M. ; Watkins, S. ; Kagan, V.E. ; Bayır, H.
• Description:
  Traumatic brain injury (TBI) leads to changes in ion fluxes, alterations in mitochondrial function, and increased generation of reactive oxygen species, resulting in secondary tissue damage. Mitochondria play important signaling roles in coordination of multiple metabolic platforms in addition to their well-known role in bioenergetics. Mitochondrial signaling strongly depends on cardiolipin (CL), a mitochondria-specific structurally unusual anionic phospholipid containing four fatty acyl chains. While our previous reports indicated that CL is selectively oxidized and presents itself as a target for the redox therapy following TBI, the topography of changes of CL in the injured brain remained to be defined. Here, we present a matrix-assisted laser desorption/ionization imaging study which reports regio-specific changes in CL, in a controlled cortical impact model of TBI in rats. Matrix-assisted laser desorption/ionization imaging revealed that TBI caused early decreases in CL in the contusional cortex, ipsilateral hippocampus, and thalamus with the most highly unsaturated CL species being most susceptible to loss. Phosphatidylinositol was the only other lipid species that exhibited a significant decrease, albeit to a lesser extent than CL. Signals for other lipids remained unchanged. This is the first study evaluating the spatial distribution of CL loss after acute brain injury. We propose that the CL loss may constitute an upstream mechanism for CL-driven signaling in different brain regions as an early response mechanism and may also underlie the bioenergetic changes that occur in hippocampal, cortical, and thalamic mitochondria after TBI.
• Keywords:
  Animals Brain Contusion Brain Injuries, Traumatic Cardiolipins Cerebral Cortex Hippocampus Male Rats Rats, Sprague-Dawley Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Thalamus

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• Source:
  J Neurochem. 139(4):659-675
• Pubmed ID:
  27591733
• Pubmed Central ID:
  PMC5323070
• Document Type:
  Journal Article
• Funding:
  R01 ES020693/ES/NIEHS NIH HHSUnited States/ ; R01 OH008282/OH/NIOSH CDC HHSUnited States/ ; U19 AI068021/AI/NIAID NIH HHSUnited States/ ; P30 CA047904/CA/NCI NIH HHSUnited States/ ; R37 NS061817/NS/NINDS NIH HHSUnited States/ ; R01 NS076511/NS/NINDS NIH HHSUnited States/ ; U54 GM103529/GM/NIGMS NIH HHSUnited States/ ; R01 NS061817/NS/NINDS NIH HHSUnited States/
• Volume:
  139
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:cb1a57613fb55772a1d3aa4aa22c0e44f9575ee1a8d35e62c0511def17d58f92
• Download URL:
  https://stacks.cdc.gov/view/cdc/44348/cdc_44348_DS1.pdf
• File Type:
  [PDF - 3.78 MB ]