Details:

Alternative Title:
ACS Chem Biol
Personal Author:
Bonger, Kimberly M. ; Rakhit, Rishi ; Payumo, Alexander Y. ; Chen, James K. ; Wandless, Thomas J.
Bonger, Kimberly M. ; Rakhit, Rishi ; Payumo, Alexander Y. ; Chen, James K. ; Wandless, Thomas J. Less -
Description:
Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.
Subjects:
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Animals Article Avena Sativa Light Luminescent Proteins Mice NIH 3T3 Cells Photolysis Phototropins Protein Engineering Protein Stability Protein Structure, Tertiary Recombinant Fusion Proteins Zebrafish
Source:
ACS Chem Biol. 9(1):111-115.
Pubmed ID:
24180414
Pubmed Central ID:
PMC3906921
Document Type:
Journal Article
Funding:
DP1 HD075622/DP/NCCDPHP CDC HHS/United States ; P50 GM107615/GM/NIGMS NIH HHS/United States ; R01 GM073046/GM/NIGMS NIH HHS/United States ; R01 GM073046/GM/NIGMS NIH HHS/United States
DP1 HD075622/DP/NCCDPHP CDC HHS/United States ; P50 GM107615/GM/NIGMS NIH HHS/United States ; R01 GM073046/GM/NIGMS NIH HHS/United States ; R01 GM073046/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:7259f802b7f853f744691ae4137ec08221dd9306a2057aa5587591aee90215d3
Download URL:
https://stacks.cdc.gov/view/cdc/29840/cdc_29840_DS1.pdf
File Type:

Supporting Files

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  	nihms-538470.nxml	txt
  	NIHMS538470-supplement-1_si_001.pdf	pdf

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