A General Method for Regulating Protein Stability with Light

Bonger, Kimberly M.; Rakhit, Rishi; Payumo, Alexander Y.; Chen, James K.; Wandless, Thomas J.

doi:10.1021/cb400755b

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A General Method for Regulating Protein Stability with Light

Published Date:

Nov 08 2013

Publisher's site:

http://dx.doi.org/10.1021/cb400755b New Site Icon

Source:

ACS Chem Biol. 9(1):111-115.

[PDF-403.86 KB]

Details:

Personal Authors:

Bonger, Kimberly M. ; Rakhit, Rishi ; Payumo, Alexander Y. ; Chen, James K. ; Wandless, Thomas J. ;

Keywords:

[+]

Pubmed ID:

24180414

Pubmed Central ID:

PMC3906921

Description:

Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Funding:

DP1 HD075622/DP/NCCDPHP CDC HHS/United States
P50 GM107615/GM/NIGMS NIH HHS/United States
R01 GM073046/GM/NIGMS NIH HHS/United States
R01 GM073046/GM/NIGMS NIH HHS/United States

Supporting Files:

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	NIHMS538470-supplement-1_si_001.pdf	application/pdf

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