A General Method for Regulating Protein Stability with Light

Details

• Alternative Title:
  ACS Chem Biol
• Personal Author:
  Bonger, Kimberly M. ; Rakhit, Rishi ; Payumo, Alexander Y. ; Chen, James K. ; Wandless, Thomas J.
• Description:
  Post-translational regulation of protein abundance in cells is a powerful tool for studying protein function. Here, we describe a novel genetically encoded protein domain that is degraded upon exposure to nontoxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.
• Subjects:
  Animals Article Avena Sativa Light Luminescent Proteins Mice NIH 3T3 Cells Photolysis Phototropins Protein Engineering Protein Stability Protein Structure, Tertiary Recombinant Fusion Proteins Zebrafish

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• Source:
  ACS Chem Biol. 9(1):111-115.
• Pubmed ID:
  24180414
• Pubmed Central ID:
  PMC3906921
• Document Type:
  Journal Article
• Funding:
  DP1 HD075622/DP/NCCDPHP CDC HHS/United States ; P50 GM107615/GM/NIGMS NIH HHS/United States ; R01 GM073046/GM/NIGMS NIH HHS/United States
• Volume:
  9
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:7259f802b7f853f744691ae4137ec08221dd9306a2057aa5587591aee90215d3
• Download URL:
  https://stacks.cdc.gov/view/cdc/29840/cdc_29840_DS1.pdf
• File Type:
  [PDF - 403.86 KB ]