A Microfluidic Paper-Based Analytical Device for Rapid Quantification of Particulate Chromium

Details

• Alternative Title:
  Anal Chim Acta
• Personal Author:
  Rattanarat, Poomrat ; Dungchai, Wijitar ; Cate, David M. ; Siangproh, Weena ; Volckens, John ; Chailapakul, Orawon ; Henry, Charles S.
• Description:
  Occupational exposure to Cr is concerning because of its myriad of health effects. Assessing chromium exposure is also cost and resource intensive because the analysis typically uses sophisticated instrumental techniques like inductively coupled plasma-mass spectrometry (ICP-MS). Here, we report a novel, simple, inexpensive microfluidic paper-based analytical device (μPAD) for measuring total Cr in airborne particulate matter. In the μPAD, tetravalent cerium (Ce(IV)) was used in a pretreatment zone to oxidize all soluble Cr to Cr(VI). After elution to the detection zone, Cr(VI) reacts with 1,5-diphenylcarbazide (1,5-DPC) forming 1,5-diphenylcarbazone (DPCO) and Cr(III). The resulting Cr(III) forms a distinct purple colored complex with the DPCO. As proof-of-principle, particulate matter (PM) collected on a sample filter was analyzed with the μPAD to quantify the mass of total Cr. A log-linear working range (0.23-3.75 μg; r(2)=0.998) between Cr and color intensity was obtained with a detection limit of 0.12 μg. For validation, a certified reference containing multiple competing metals was analyzed. Quantitative agreement was obtained between known Cr levels in the sample and the Cr measured using the μPAD.
• Subjects:
  Cerium Chromium Colorimetry Diphenylcarbazide Hydrogen-Ion Concentration Microfluidic Analytical Techniques Microwaves Nitric Acid Oxidation-Reduction Paper Particulate Matter Polyethylenes Quaternary Ammonium Compounds

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• Source:
  Anal Chim Acta. 2013; 800:50-55
• Pubmed ID:
  24120167
• Pubmed Central ID:
  PMC3842604
• Document Type:
  Journal Article
• Funding:
  R01 EB004876/EB/NIBIB NIH HHSUnited States/ ; R21 OH010050/OH/NIOSH CDC HHSUnited States/ ; R21OH010050/OH/NIOSH CDC HHSUnited States/
• Volume:
  800
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:5622067e542d4af7715af83f4b5ce07bbf70b9657a1dd56a2c83c1af725def7e09a11049ac904e550896300ecf7f367cda890865b9b3c9c5179126169bd7949f
• Download URL:
  https://stacks.cdc.gov/view/cdc/29789/cdc_29789_DS1.pdf
• File Type:
  [PDF - 1.70 MB ]