“Dip-and-read” paper-based analytical devices using distance-based detection with color screening

Details

• Alternative Title:
  Lab Chip
• Personal Author:
  Yamada, Kentaro ; Citterio, Daniel ; Henry, Charles S.
• Description:
  An improved paper-based analytical device (PAD) using color screening to enhance device performance is described. Current detection methods for PADs relying on the distance-based signalling motif can be slow due to the assay time being limited by capillary flow rates that wick fluid through the detection zone. For traditional distance-based detection motifs, analysis can take up to 45 min for a channel length of 5 cm. By using a color screening method, quantification with a distance-based PAD can be achieved in minutes through a "dip-and-read" approach. A colorimetric indicator line deposited onto a paper substrate using inkjet-printing undergoes a concentration-dependent colorimetric response for a given analyte. This color intensity-based response has been converted to a distance-based signal by overlaying a color filter with a continuous color intensity gradient matching the color of the developed indicator line. As a proof-of-concept, Ni quantification in welding fume was performed as a model assay. The results of multiple independent user testing gave mean absolute percentage error and average relative standard deviations of 10.5% and 11.2% respectively, which were an improvement over analysis based on simple visual color comparison with a read guide (12.2%, 14.9%). In addition to the analytical performance comparison, an interference study and a shelf life investigation were performed to further demonstrate practical utility. The developed system demonstrates an alternative detection approach for distance-based PADs enabling fast (∼10 min), quantitative, and straightforward assays.
• Subjects:
  Colorimetry Environmental Pollutants Equipment Design Microfluidic Analytical Techniques Nickel Paper
• Source:
  Lab Chip. 18(10):1485-1493
• Pubmed ID:
  29693672
• Pubmed Central ID:
  PMC5975359
• Document Type:
  Journal Article
• Funding:
  R01 OH010662/OH/NIOSH CDC HHSUnited States/ ; R01OH010662/ACL HHSUnited States/
• Volume:
  18
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:06413852767927fd6101f878940766d1ec0438cb37ec8a43eb435d5b488a8ce6
• Download URL:
  https://stacks.cdc.gov/view/cdc/55378/cdc_55378_DS1.pdf
• File Type:
  [PDF - 2.29 MB ]