Phage-Displayed Peptides that Mimic Aflatoxins and its Application in Immunoassay

Details

• Alternative Title:
  J Agric Food Chem
• Personal Author:
  Wang, Yanru ; Wang, Hong ; Li, Peiwu ; Zhang, Qi ; Kim, Hee Joo ; Gee, Shirley J. ; Hammock, Bruce D.
• Description:
  To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC50 value of 14 μg/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 μg/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis.
• Subjects:
  Aflatoxin Aflatoxins Arachis Article Enzyme-Linked Immunosorbent Assay Food Contamination Matrix Effect Mimotope Oryza Peptide Library Peptides Phage-displayed Peptide Phage Peptide Library Zea Mays

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• Source:
  J Agric Food Chem. 61(10):2426-2433
• Pubmed ID:
  23394544
• Pubmed Central ID:
  PMC3830680
• Document Type:
  Journal Article
• Funding:
  U50 OH007550/OH/NIOSH CDC HHSUnited States/ ; P42 ES004699/ES/NIEHS NIH HHSUnited States/ ; P42 ES04699/ES/NIEHS NIH HHSUnited States/ ; U54 NS079202/NS/NINDS NIH HHSUnited States/ ; U50 OH07550/OH/NIOSH CDC HHSUnited States/
• Volume:
  61
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:b18ef00ca7c2b79949be81c2a816660656b4c9b5f7b9cd558d2e28ad07398f9f
• Download URL:
  https://stacks.cdc.gov/view/cdc/29775/cdc_29775_DS1.pdf
• File Type:
  [PDF - 2.65 MB ]