Correction to Phage-Displayed Peptide That Mimics Aflatoxins and Its Application in Immunoassay

Details

• Personal Author:
  Gee, Shirley J. ; Hammock, Bruce D. ; Kim, Hee Joo ; Li, Peiwu ; Wang, Hong ; Wang, Yanru ; Zhang, Qi
• Description:
  To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC50 value of 14 µg/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 µg/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis. Erratum: J Agric Food Chem 2013 Apr; 61(16):3994 [Description provided by NIOSH]
• Subjects:
  Fungi Hazardous Substances Microbiology Occupational Health Safety
• Keywords:
  Analytical-processes; Fungi; Toxins; Microorganisms; Author Keywords: Aflatoxin; Phage-displayed Peptide; Phage Peptide Library; Mimotope; Matrix Effect
• ISSN:
  0021-8561
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1 pdf page
• Volume:
  60
• NIOSHTIC Number:
  nn:20044636
• Citation:
  J Agric Food Chem 2013 Mar; 61(10):2426-2433
• Contact Point Address:
  Peiwu Li, Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, 430062, P. R. China
• Email:
  peiwuli@oilcrops.cn
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Agricultural and Food Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f6e0df4f1a3517e1ddbffd5ddd025aac6833f32cb7bab3b174d9d4946b0dfec3f4c0a503b715e5e4eca47c1630115369438b17522feb91b5fb3bfd25f5fd8cbe
• Download URL:
  https://stacks.cdc.gov/view/cdc/231607/cdc_231607_DS1.pdf
• File Type:
  [PDF - 109.81 KB ]