The Fumagillin Biosynthetic Gene Cluster in Aspergillus fumigatus Encodes a Cryptic Terpene Cyclase Involved in the Formation of β-trans-Bergamotene

Lin, Hsiao-Ching; Chooi, Yit-Heng; Dhingra, Sourabh; Xu, Wei; Calvo, Ana M.; Tang, Yi

doi:10.1021/ja312503y

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The Fumagillin Biosynthetic Gene Cluster in Aspergillus fumigatus Encodes a Cryptic Terpene Cyclase Involved in the Formation of β-trans-Bergamotene

Published Date:

Mar 19 2013

Publisher's site:

http://dx.doi.org/10.1021/ja312503y

Source:

J Am Chem Soc. 135(12):4616-4619.

[PDF-913.70 KB]

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Alternative Title:

J Am Chem Soc

Personal Author:

Lin, Hsiao-Ching ; Chooi, Yit-Heng ; Dhingra, Sourabh ; Xu, Wei ; Calvo, Ana M. ; ... More ▼

Description:

Fumagillin 1 is a meroterpenoid from Aspergillus fumigatus that is known for its anti-angiogenic activity by binding to human methionine aminopeptidase 2. The genetic and molecular basis for biosynthesis of 1 had been an enigma despite the availability of the A. fumigatus genome sequence. Here, we report the identification and verification of the fma gene cluster, followed by characterization of the polyketide synthase and acyltransferase involved in biosynthesis of the dioic acid portion of 1. More significantly, we uncovered the elusive β-trans-bergamotene synthase in A. fumigatus as a membrane-bound terpene cyclase.

Subject:

[+]

Pubmed ID:

23488861

Pubmed Central ID:

PMC3652892

Document Type:

Journal Article

Funding:

DP1 GM106413/GM/NIGMS NIH HHS/United States ; R01 GM085128/GM/NIGMS NIH HHS/United States ; 1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1R01GM085128/GM/NIGMS NIH HHS/United States ;

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CDC Public Access

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The Fumagillin Biosynthetic Gene Cluster in Aspergillus fumigatus Encodes a Cryptic Terpene Cyclase Involved in the Formation of β-trans-Bergamotene

The Fumagillin Biosynthetic Gene Cluster in Aspergillus fumigatus Encodes a Cryptic Terpene Cyclase Involved in the Formation of β-trans-Bergamotene

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