Details:

Alternative Title:
J Am Chem Soc
Personal Author:
Lin, Hsiao-Ching ; Chooi, Yit-Heng ; Dhingra, Sourabh ; Xu, Wei ; Calvo, Ana M. ; ... More +
Lin, Hsiao-Ching ; Chooi, Yit-Heng ; Dhingra, Sourabh ; Xu, Wei ; Calvo, Ana M. ; Tang, Yi Less -
Description:
Fumagillin 1 is a meroterpenoid from Aspergillus fumigatus that is known for its anti-angiogenic activity by binding to human methionine aminopeptidase 2. The genetic and molecular basis for biosynthesis of 1 had been an enigma despite the availability of the A. fumigatus genome sequence. Here, we report the identification and verification of the fma gene cluster, followed by characterization of the polyketide synthase and acyltransferase involved in biosynthesis of the dioic acid portion of 1. More significantly, we uncovered the elusive β-trans-bergamotene synthase in A. fumigatus as a membrane-bound terpene cyclase.
Subjects:
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Article Aspergillus Fumigatus Cyclohexanes Fatty Acids, Unsaturated Genes, Fungal Genome Mining Meroterpenoids Multigene Family Polyketide Polyketide Synthases Prenyltransferase Sesquiterpenes Terpene Cyclase
Source:
J Am Chem Soc. 135(12):4616-4619
Pubmed ID:
23488861
Pubmed Central ID:
PMC3652892
Document Type:
Journal Article
Funding:
DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/
DP1 GM106413/GM/NIGMS NIH HHSUnited States/ ; R01 GM085128/GM/NIGMS NIH HHSUnited States/ ; 1DP1GM106413/DP/NCCDPHP CDC HHSUnited States/ ; 1R01GM085128/GM/NIGMS NIH HHSUnited States/ Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:fccc7737204b1cc529187e1a48bbb5e0b26158801f851bf69f2cc4051d500f26
Download URL:
https://stacks.cdc.gov/view/cdc/29658/cdc_29658_DS1.pdf
File Type:

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