Details:

Alternative Title:
J Am Chem Soc
Publisher's site:
http://dx.doi.org/10.1021/ja312503y
Personal Author:
Lin, Hsiao-Ching ; Chooi, Yit-Heng ; Dhingra, Sourabh ; Xu, Wei ; Calvo, Ana M. ; ... More +
Lin, Hsiao-Ching ; Chooi, Yit-Heng ; Dhingra, Sourabh ; Xu, Wei ; Calvo, Ana M. ; Tang, Yi ; Less -
Description:
Fumagillin 1 is a meroterpenoid from Aspergillus fumigatus that is known for its anti-angiogenic activity by binding to human methionine aminopeptidase 2. The genetic and molecular basis for biosynthesis of 1 had been an enigma despite the availability of the A. fumigatus genome sequence. Here, we report the identification and verification of the fma gene cluster, followed by characterization of the polyketide synthase and acyltransferase involved in biosynthesis of the dioic acid portion of 1. More significantly, we uncovered the elusive β-trans-bergamotene synthase in A. fumigatus as a membrane-bound terpene cyclase.
Subject:
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Article
Aspergillus Fumigatus
Cyclohexanes
Fatty Acids, Unsaturated
Genes, Fungal
Genome Mining
Meroterpenoids
Multigene Family
Polyketide
Polyketide Synthases
Prenyltransferase
Sesquiterpenes
Terpene Cyclase

Source:
J Am Chem Soc. 135(12):4616-4619. ;
Pubmed ID:
23488861
Pubmed Central ID:
PMC3652892
Document Type:
Journal Article ;
Funding:
DP1 GM106413/GM/NIGMS NIH HHS/United States ; R01 GM085128/GM/NIGMS NIH HHS/United States ; 1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1R01GM085128/GM/NIGMS NIH HHS/United States ;
DP1 GM106413/GM/NIGMS NIH HHS/United States ; R01 GM085128/GM/NIGMS NIH HHS/United States ; 1DP1GM106413/DP/NCCDPHP CDC HHS/United States ; 1R01GM085128/GM/NIGMS NIH HHS/United States ; Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:fccc7737204b1cc529187e1a48bbb5e0b26158801f851bf69f2cc4051d500f26
File Type:

Supporting Files

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