Optical clearing-aided photoacoustic microscopy with enhanced resolution and imaging depth

Details

• Alternative Title:
  Opt Lett
• Personal Author:
  Zhou, Yong ; Yao, Junjie ; Wang, Lihong V.
• Description:
  Due to strong light scattering in tissue, both the spatial resolution and maximum penetration depth of optical-resolution photoacoustic microscopy (OR-PAM) deteriorate sharply with depth. To reduce tissue scattering, we propose to use glycerol as an optical clearing agent in OR-PAM. Our results show that the imaging performance of OR-PAM can be greatly enhanced by optical clearing both in vitro and in vivo.
• Subjects:
  Animals Article Epidermis Image Processing, Computer-Assisted Mice Microscopy Optical Phenomena Phantoms, Imaging Photoacoustic Techniques Signal-To-Noise Ratio
• Source:
  Opt Lett. 2013; 38(14):2592-2595.
• Pubmed ID:
  23939121
• Pubmed Central ID:
  PMC3759812
• Document Type:
  Journal Article
• Funding:
  T32 EB014855/EB/NIBIB NIH HHS/United States ; R01 CA134539/CA/NCI NIH HHS/United States ; R01 CA157277/CA/NCI NIH HHS/United States ; U54 CA136398/CA/NCI NIH HHS/United States ; R01 CA159959/CA/NCI NIH HHS/United States ; DP1 EB016986/EB/NIBIB NIH HHS/United States ; R01 EB008085/EB/NIBIB NIH HHS/United States ; DP1 EB016986/DP/NCCDPHP CDC HHS/United States
• Volume:
  38
• Issue:
  14
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:036828e61a4153f8427de792c7306810594ccbdf0c9aefdc92c8ee3d83e6f5ce
• Download URL:
  https://stacks.cdc.gov/view/cdc/29721/cdc_29721_DS1.pdf
• File Type:
  [PDF - 1.15 MB ]