Temporal Changes in Resistance Mechanisms in Colonizing Escherichia coli Isolates with Reduced Susceptibility to Fluoroquinolones

Details

• Alternative Title:
  Diagn Microbiol Infect Dis
• Personal Author:
  Han, Jennifer H. ; Nachamkin, Irving ; Tolomeo, Pam ; Mao, Xiangqun ; Bilker, Warren B. ; Lautenbach, Ebbing
• Description:
  The objective of this study was to characterize the temporal variability of fluoroquinolone resistance mechanisms among Escherichia coli colonizing the gastrointestinal tract of hospitalized patients. Patients with new fluoroquinolone-resistant E. coli (FQREC) colonization were followed with serial fecal sampling until discharge or death. Genetic mechanism(s) of resistance for all FQREC isolates was characterized, including mutations in gyrA and parC and efflux pump overexpression. Of 451 subjects, 73 (16.2%) became newly colonized with FQREC. There was significant variability in regard to temporal changes in resistance mechanisms and levofloxacin MICs among isolates from individual patients. Compared to patients with transient colonization, patients with persistent colonization were more likely to have a urinary catheter (P = 0.04), diarrhea (P = 0.04), and a longer duration of hospitalization (22 and 9.0 mean days, respectively; P = 0.01) prior to sampling. Our data demonstrate the significant variability of resistance mechanisms in colonizing E. coli isolates among hospitalized patients.
• Keywords:
  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA Gyrase
  • DNA Topoisomerase IV
  • Drug Resistance, Bacterial
  • Escherichia coli
  • Escherichia coli Infections
  More +
• Source:
  Diagn Microbiol Infect Dis. 2013; 76(4):491-496
• Pubmed ID:
  23719087
• Pubmed Central ID:
  PMC3725193
• Document Type:
  Journal Article
• Funding:
  K24 AI080942/AI/NIAID NIH HHSUnited States/ ; U54CK000163/ACL/ACL HHSUnited States/ ; ImNIH/Intramural NIH HHSUnited States/ ; RS1/CCR320627-01/RS/DRS NIH HHSUnited States/ ; U54-CK000163/CK/NCEZID CDC HHSUnited States/
• Volume:
  76
• Issue:
  4
• Download URL:
  https://stacks.cdc.gov/view/cdc/29695/cdc_29695_DS1.pdf
• File Type:
  [PDF - 384.09 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:999773ef6c2121c8db4e3779417c8965f81650e7c0f3e9e8305ec8b691c9dbeb