Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses

Zhao, Jiangqin; Ragupathy, Viswanath; Liu, Jikun; Wang, Xue; Vemula, Sai Vikram; El Mubarak, Haja Sittana; Ye, Zhiping; Landry, Marie L.; Hewlett, Indira

doi:10.3201/eid2103.141169

i

Nanomicroarray and Multiplex Next-Generation Sequencing for Simultaneous Identification and Characterization of Influenza Viruses

Supporting Files Public Domain

Mar 2015
By Zhao, Jiangqin ; Ragupathy, Viswanath ; Liu, Jikun ; ...

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Zhao, Jiangqin ; Ragupathy, Viswanath ; Liu, Jikun ; Wang, Xue ; Vemula, Sai Vikram ; El Mubarak, Haja Sittana ; Ye, Zhiping ; Landry, Marie L. ; Hewlett, Indira
Description:

Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics.
Subjects:

DNA Genes Hemagglutinin Influenza Influenza A Influenza Virus Nanomicroarray Nanomicroarray And Multiplex Next-Generation Sequencing For Simultaneous Identification And Characterization Of Influenza Viruses Neuraminidase Next-generation Sequencing Research RNA Subtype Viruses
Source:

Emerg Infect Dis. 21(3):400-408.
Document Type:

Journal Article
Volume:

21
Issue:

3
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:4e22a25e6bfe94261a4d3e6b17fa1daee100e736920afc33780e5cf7da8eafb8
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https://stacks.cdc.gov/view/cdc/28101/cdc_28101_DS1.pdf
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[PDF - 700.76 KB ]

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