Details:

Alternative Title:
Emerg Infect Dis
Personal Author:
Zhao, Jiangqin ; Ragupathy, Viswanath ; Liu, Jikun ; Wang, Xue ; Vemula, Sai Vikram ; ... More +
Zhao, Jiangqin ; Ragupathy, Viswanath ; Liu, Jikun ; Wang, Xue ; Vemula, Sai Vikram ; El Mubarak, Haja Sittana ; Ye, Zhiping ; Landry, Marie L. ; Hewlett, Indira Less -
Description:
Conventional methods for detection and discrimination of influenza viruses are time consuming and labor intensive. We developed a diagnostic platform for simultaneous identification and characterization of influenza viruses that uses a combination of nanomicroarray for screening and multiplex next-generation sequencing (NGS) assays for laboratory confirmation. The nanomicroarray was developed to target hemagglutinin, neuraminidase, and matrix genes to identify influenza A and B viruses. PCR amplicons synthesized by using an adapted universal primer for all 8 gene segments of 9 influenza A subtypes were detected in the nanomicroarray and confirmed by the NGS assays. This platform can simultaneously detect and differentiate multiple influenza A subtypes in a single sample. Use of these methods as part of a new diagnostic algorithm for detection and confirmation of influenza infections may provide ongoing public health benefits by assisting with future epidemiologic studies and improving preparedness for potential influenza pandemics.
Subjects:
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DNA Genes Hemagglutinin Influenza Influenza A Influenza Virus Nanomicroarray Nanomicroarray And Multiplex Next-Generation Sequencing For Simultaneous Identification And Characterization Of Influenza Viruses Neuraminidase Next-generation Sequencing Research RNA Subtype Viruses
Source:
Emerg Infect Dis. 21(3):400-408.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:4e22a25e6bfe94261a4d3e6b17fa1daee100e736920afc33780e5cf7da8eafb8
Download URL:
https://stacks.cdc.gov/view/cdc/28101/cdc_28101_DS1.pdf
File Type:

Supporting Files

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