Skilled Nursing Facility Wastewater Surveillance: a SARS-CoV-2 and Antimicrobial Resistance Detection Pilot Study.

Details

• Alternative Title:
  Journal of Water and Health, 2025, v. 23, no. 6: Skilled Nursing Facility Wastewater Surveillance: a SARS-CoV-2 and Antimicrobial Resistance Detection Pilot Study.
• Journal Article:
  Journal of Water and Health
• Personal Author:
  Santiago, Ariel Jose ; Garay, Maria Burgos ; Campbell, Mariya ; Cahela, Yimu ; Donlan, Rodney ; Gable, Paige ; Kyros, Christine Ganim ; Franco, Lauren ; Kartforosh, Leila ; Lenz, Susanna ; Lyons, Amanda K ; Moore, Jamari ; Noble-Wang, Judith ; Sanders, Carrie ; Abera, Bethelhem ; Adler, Colin H. ; Jones, Sophie ; Medrzycki, Magdalena ; Walters, Maroya S. ; Cook, Peter ; Li, Yan ; Tao, Ying ; Zhang, Jing ; Malapati, Lakshmi ; Retchless, Adam ; Tong, Suxiang ; Coulliette-Salmond, Angela D.
• Description:
  The purpose of this study was to determine the feasibility of facility-level wastewater surveillance in the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in skilled nursing facility (SNF) wastewater using three concentration methods, as well as a proof-of-concept for antimicrobial resistance (AR) genes/organisms detection. Wastewater effluent samples were collected from an SNF over an 8-week period. Wastewater was concentrated using electronegative membrane filtration (enMF), polyethylene glycol precipitation, and Nanotrap
  
  magnetic virus particles (NP). Quantification of the genome copy concentration from SARS-CoV-2 and bovine respiratory syncytial virus (BRSV), a SARS-CoV-2 surrogate spiked into all samples, was performed with droplet digital polymerase chain reaction (ddPCR). Wastewater sample aliquots were also enriched in microbiological culture media and screened for organisms with AR phenotypes on selective and differential agars. Multiplex real-time PCR was used to detect a broad array of carbapenem resistance genes. SARS-CoV-2 was detected and quantified from a single enMF-concentrated wastewater sample. The highest concentration of BRSV came from enMF-concentrated samples.
  
  ,
  
  ,
  
  , and
  
  exhibiting AR phenotypes were successfully detected using culture-dependent approaches. Culture-independent, multiplex PCR indicated that bla
  
  was the main carbapenemase gene detected in wastewater samples. Facility-level wastewater surveillance could be a useful strategy for SNFs.
• Content Notes:
  Author manuscript
• Subjects:
  • Wastewater
  • Skilled Nursing Facilities
  • Pilot Projects
  • SARS-CoV-2
  • COVID-19
  • Humans
  • Environmental Monitoring
  • Drug Resistance, Bacterial
• Keywords:
  • antimicrobial resistance
  • SARS-CoV-2
  • skilled nursing facility
  • wastewater surveillance
• Source:
  Journal of Water and Health, 2025, v. 23, no. 6
• DOI:
  https://doi.org/10.2166/wh.2025.374
• ISSN:
  1477-8920 ; 1996-7829
• Pubmed ID:
  40586337
• Pubmed Central ID:
  PMC12323735
• Document Type:
  Text
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Genre:
  Journal Article
• Pages in Document:
  727-742 (25 pdf pages)
• Volume:
  23
• Issue:
  6
• Download URL:
  https://stacks.cdc.gov/view/cdc/261291/cdc_261291_DS1.pdf
• File Type:
  [PDF - 696.18 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:885eded58618c9519d63d95cc8679aa0d368b877afc99fbd9734ceacc356331c14cc01c278157fd1ccd1913df742dc126196e12663ec1206f31a9eebfb4cb70a