Lung Toxicity Profile of Inhaled Copper-Nickel Welding Fume in A/J Mice (Dataset)

Details

• Corporate Authors:
  National Institute for Occupational Safety and Health
• Description:
  The process of stainless steel welding creates fumes rich in carcinogenic metals such as chromium (Cr). Welding consumables devoid of Cr are being produced in attempt to limit worker exposures to potentially toxic and carcinogenic metals. The study objective was to characterize a copper-nickel (Cu-Ni) fume generated using gas metal arc welding (GMAW) and determine the pulmonary deposition and toxicity of the fume in mice exposed by inhalation. Male A/J mice (6 - 8 weeks of age) were exposed to air or Cu-Ni welding fumes for 2 (low deposition) or 4 (high deposition) hours/day for 10 days. Mice were sacrificed and bronchoalveolar lavage (BAL), macrophage function, and histopathological analysis were performed at various timepoints post-exposure to evaluate resolution. Characterization of the fume indicated that most of the particles were between 0.1 and 1 µm in diameter, with a mass median aerodynamic diameter of 0.43 µm. Metal content of the fume was primarily Cu (approx. 76%) and Ni (approx. 12%). After exposure, BAL macrophages had a reduced ability to phagocytose E. coli at 1 and 7 days and lung cytotoxicity was evident and significant (>12-19% fold change). Loss of body weight was also significant at these two early timepoints. Lung inflammation, the predominant lesion identified by histopathology, was observed as a subacute response early that progressively resolved by 28 days with only macrophage aggregates remaining late (84 days). Future studies are planned to investigate the tumorigenic potential of Cu-Ni fume in A/J mice. [Description provided by NIOSH]
• Subjects:
  Animals Blood Chromium Lung Macrophages Metals Nickel Occupational Health Respiratory System Safety Welding

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• Keywords:
  Animal studies; Toxic effects; Lung tissue; Welding fumes; Stainless steel; Metals; Chromium; Copper alloys; Nickel; Macrophages
• DOI:
  https://doi.org/10.26616/NIOSHRD-1040-2022-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20065527
• Citation:
  Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1040-2022-0, 2022 Jul; :dataset
• Contact Point Address:
  Pathology and Physiology Research Branch (PPRB), Health Effects Laboratory Division (HELD), National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV. Tel: 304-285-5831
• CAS Registry Number:
  Chromium (CAS RN 7440-47-3) ; Copper (CAS RN 7440-50-8) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2022
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  Lung toxicity profile of inhaled copper-nickel welding fume in A/J mice
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:e110d641223a7f7e2b59f8980c6d9902f1ea45a1c5860300828a8f46bc80fae47a6bfe891226165fa2f25eaa4730b2c426cc19ae2258c6dbe0f75db0ecd5d2e1
• Download URL:
  https://stacks.cdc.gov/view/cdc/248913/cdc_248913_DS1.pdf
• File Type:
  [PDF - 141.49 KB ]