Effects of Silica Inhalation on Metabolic Obesity in a Western Diet-Induced F344 Rat Model; Silica Inhibits Adipose Function and Diet-Induced Inflammation (Dataset)

Details

• Corporate Authors:
  National Institute for Occupational Safety and Health
• Description:
  Introduction: Adipose tissue (AT), an endocrine organ, plays a central role in maintenance of whole-body energy homeostasis through its release of adipokines. Obesity, affecting over 40% of American adults, disrupts adipocyte metabolism leading to chronic systemic inflammation and metabolic dysfunction (MetDys). MetDys is associated with impaired lung function, pulmonary hypertension, and asthma. The aim of this study was to investigate the effects of silica inhalation in a pre-existing MetDys animal model to determine if an occupational exposure of silica dust has the potential to initiate or further the progression of MetDys associated conditions. Methods Collection: 1) This laboratory-based investigation studied the effects of respirable crystalline silica on metabolic function in a high fat western diet (HFWD)-induced MetDys F344 rat model. 2) Six wk old male rats were fed a HFWD or standard (STD; control) for 16 wk prior to inhalation exposure to respirable crystalline silica dust (6 h /d, 5 d / wk, 39 d) with end point measurements taken at 0, 4 and 8 wk post-exposure to silica. 3) Animals were maintained on their assigned diet for the duration of the study. 4) Repeated measures of fasting glucose and laser-Doppler flowmetry were made prior to exposure and at 0, 4, and 8 wk using the same cohort of animals. Animals were fasted 12 h overnight for fasting glucose measurement; blood glucose was measured using a hand-held glucometer. Rat tail arterial function and blood flow was measured for 15 minutes using the non-invasive laser-Doppler flowmetry. 5) Endpoint measurements taken made following removal of tissues/organs from euthanized animals in in vitro studies. Endpoint measurements included: Anthropometric measures of weight, length, abdominal girth, ependymal fat pad weight; Measurement of serum insulin, leptin and adiponectin hormones and blood clinical chemistry; and Hematology measures. [Description provided by NIOSH]
• Subjects:
  Animals Dust Energy Metabolism Occupational Health Respiratory System Safety Silicon Dioxide
• Keywords:
  Adipose Tissue Animal Studies Crystalline Silica Dietary Effects Endocrine System Inhalation Studies Metabolic Disorders Metabolic Effects Obesity Respirable Dust Silica Dusts

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• DOI:
  https://doi.org/10.26616/NIOSHRD-1019-2021-0
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Dataset
• Genre:
  Research Dataset
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• NIOSHTIC Number:
  nn:20063037
• Citation:
  Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Research Dataset RD-1019-2021-0, 2021 Jul; :dataset
• Contact Point Address:
  Pathology and Physiology Research Branch (PPRB), Health Effects Lab Division (HELD), National Institute for Occupational Safety and Health (NIOSH), Morgantown, WV, 304.285.5831
• CAS Registry Number:
  Quartz (SiO2) (CAS RN 14808-60-7)
• Federal Fiscal Year:
  2021
• NORA Priority Area:
  Construction ; Mining
• Peer Reviewed:
  False
• Source Full Name:
  Effects of silica inhalation on metabolic obesity in a western diet-induced F344 rat model; silica inhibits adipose function and diet-induced inflammation
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:231115f7e8a1d50a4eefade6ea30daaa5ccc5197c2383812977df769ed5265c25d8d79786a6622789bc0eb5ce1b82fe8fc66e8c4737bc82eade4048342bc3f4d
• Download URL:
  https://stacks.cdc.gov/view/cdc/226167/cdc_226167_DS1.pdf
• File Type:
  [PDF - 169.52 KB ]