Comparative study of micronucleus assay and chromosomal aberration analysis in V79 cells exposed to ethylene oxide.

Details

• Personal Author:
  Gu Z ; Ong T ; Wallace WE ; Whong W ; Zhong B
• Description:
  Induction of micronuclei and chromosome aberrations in hamster lung fibroblasts by ethylene-oxide (75218) was examined. Chinese-hamster- V79 lung fibroblasts were cultured and exposed to 3500, 6900, 13,800, or 27,700 parts per million (ppm) ethylene-oxide for 30 minutes. The cells were examined for chromosome aberrations. Other V79 cell cultures were exposed to 457, 1372, 4115, or 12344ppm ethylene-oxide for 30 minutes. The cells were then treated with 3 micrograms per milliliter cytochalasin-B (CytB). The cells were scored for micronuclei. The proportion of binucleated cells was determined. Ethylene-oxide induced significant dose dependent increases in chromosome aberration frequency. The aberrations consisted of chromatid and isochromatid breaks, fragments, minutes, and exchanges. Ethylene-oxide increased the frequency of micronucleated V79 cells; however, only the increase induced by the 12,344ppm exposure was statistically significant. The increases were approximately 1.5 to 3 times higher in cells that had been treated with CytB. The proportion of binucleated cells was increased by CytB but decreased by ethylene-oxide. The decrease induced by ethylene-oxide was dose dependent. The authors conclude that the chromosome aberration and micronuclei induction data indicate that ethylene-oxide is a clastogen. Chromosome aberration analysis appears to be a more sensitive technique for detecting clastogenicity than micronuclei analysis. [Description provided by NIOSH]
• Subjects:
  Cell Division Chromosomes Epoxy Compounds Lung Occupational Health Respiratory System Safety
• Keywords:
  Author Keywords: Chromosomal Aberrations; Cytochalasin B; Micronucleus Formation; Chromatid And Isochromatid Breaks; Mononucleated And Binucleated Cells NIOSH-Author; In-vitro-studies; Epoxides; Lung-cells; Chromosome-disorders; Dose-response; Clastogens; Cell-division; Mammalian-cells;
• ISSN:
  0270-3211
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  227-233
• Volume:
  11
• Issue:
  5
• NIOSHTIC Number:
  nn:00207781
• Citation:
  Teratog, Carcinog, Mutagen 1991 Jan; 11(5):227-233
• Contact Point Address:
  Tong-Man Ong, Division of Respiratory Disease Studies, National Institute for Occupational Safety and Health, 944 Chestnut Ridge Road, Morgantown, WV 26505-2888
• CAS Registry Number:
  Ethylene oxide (CAS RN 75-21-8)
• Federal Fiscal Year:
  1991
• Peer Reviewed:
  True
• Source Full Name:
  Teratogenesis, Carcinogenesis, and Mutagenesis
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247772/cdc_247772_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]