Circadian organization of thirteen liver and six brain enzymes of the mouse.

Details

• Personal Author:
  Casciano DA ; Feuers RJ ; North C ; Pauly JE ; Scheving LE ; Tsai TH
• Description:
  Studies were conducted in mice to to determine the effects of circadian organization on 13 liver and six brain enzymes. Male CD2F1-mice were standardized either to 12 hours of light alternating with 12 hours of darkness or to the reversed dark/light cycle for 2 weeks. Mice in one group were 7 weeks old, while those in another group were 12 weeks old. Mice were killed at 3 hour intervals over a period of 2 days; brains and livers were removed and analyzed for enzymes. All 19 enzymes demonstrated a prominent circadian rhythm in at least one experiment. A statistically significant fit was noted by each rhythmic variable to a 24 hour cosine curve by the method of least squares. Liver enzymes showed their peak activities at the beginning of the dark cycle and initiation of the animal's activity. Brain enzymes peaked in activity at the beginning of the rest span and near the beginning of the light cycle. The beginning of the dietary intake process in the rodent normally occurs at the onset of darkness, and this may account for the clustering of the acrophases in enzyme activity at the beginning of the dark span. The phasing of each of the rhythms could be reversed within a 2 week period after reversing the environmental light/dark cycle by 180 degrees. The authors suggest this is the first study to simultaneously consider, from a chronobiological viewpoint, more than one or a few enzymes in the same animals in a single experiment. [Description provided by NIOSH]
• Subjects:
  Circadian Rhythm Digestive System Laboratories Liver Nervous System Occupational Health Periodicity Safety
• Keywords:
  NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Laboratory-animals; Biological-rhythms; Circadian-rhythms; Liver-enzymes; Central-nervous-system
• ISSN:
  0002-9106
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Arkansas ; OSHA Region 6
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  183-199
• Volume:
  162
• Issue:
  3
• NIOSHTIC Number:
  nn:00185754
• Citation:
  Am J Anat 1981 Nov; 162(3):183-199
• Contact Point Address:
  Anatomy University of Arkansas 4301 West Markham Little Rock, Ark 72201
• Federal Fiscal Year:
  1982
• NORA Priority Area:
  Neurotoxic Disorders ; Neurotoxic Effects
• Performing Organization:
  University of Arkansas Med Scis Ltl Rock, Little Rock, Arkansas
• Peer Reviewed:
  True
• Start Date:
  19791201
• Source Full Name:
  American Journal of Anatomy
• End Date:
  19880301
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247663/cdc_247663_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]