Involvement of the sodium-potassium pump in the mechanism of postjunctional supersensitivity of the vas deferens of the guinea pig.

Details

• Personal Author:
  Fedan, Jeffery S. ; Fleming WW ; Gerthoffer WT ; Goto K ; Westfall DP
• Description:
  The relationship between the electrogenic sodium-potassium (NaK) pump and postjunctional supersensitivity of guinea-pig vas deferens was studied. The vasa deferentia were removed from male guinea- pigs, desheathed, and exposed to ouabain (630604) followed by methoxamine (390283). Pairs of vasa deferentia were either unoperated or surgically denervated. The effects of ouabain or reduced extracellular potassium were determined. Nuclear, mitochondrial, microsomal, and supernatant fractions of homogenized vasa deferentia were assayed for NaK adenosinetriphosphatase (ATPase). Neither ouabain nor reduced potassium depolarized the cell membranes of denervated vasa deferentia, nor did ouabain enhance methoxamine sensitivity. Denervation of the vasa deferentia resulted in reduced ATPase in the subcellular fractions. The authors suggest that interruption of the excitatory innervation of the vas deferens partially inhibits the NaK pump by reducing ATPase activity. Inhibition of the pump may cause depolarization of the cell membrane due to the electrogenicity of the pump. [Description provided by NIOSH]
• Subjects:
  Enzymes Laboratories Occupational Health Safety Urogenital System
• Keywords:
  NIOSH-Author; Laboratory-animals; Enzyme-activity; Cell-biology; Cellular-reactions; Reproductive-system
• ISSN:
  0022-3565
• Document Type:
  Text
• Genre:
  Journal Article
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  27-36
• Volume:
  210
• Issue:
  1
• NIOSHTIC Number:
  nn:00118624
• Citation:
  J Pharmacol Exp Ther 1979 Jul; 210(1):27-36
• CAS Registry Number:
  Methoxamine (CAS RN 390-28-3) ; Ouabain (CAS RN 630-60-4)
• Federal Fiscal Year:
  1979
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Pharmacology and Experimental Therapeutics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/247517/cdc_247517_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]