In vivo and in vitro evaluations of spermatotoxicity induced by 2-ethoxyethanol treatment.

Details

• Personal Author:
  Oudiz D ; Zenick H
• Description:
  The mechanism of 2-ethoxyethanol (110805) (EE) action on male reproductive system was studied in-vivo and in-vitro. Male Long- Evans hooded rats were given 0 or 936 milligram/kilogram of EE by gavage, 5 days/week for 6 weeks. The initial sperm count was 60 million. The count decreased 30 to 40 percent after 5 weeks. By the sixth week, three of ten treated rats were azoospermic and the remaining rats had sperm counts between 5 and 30 million. Test animals showed abnormal sperm morphology, but had about the same motility as the control group. Testes, epididymides, and caudae epididymides weights were lower in the treated animals. The treatment of spermatocytes with ethoxyacetic-acid (627032) (EAA), the reported active metabolite of EE, elicited a significant increase in the lactate:endogeneous ratio and a significant decrease in the 2,4-dinitrophenol:lactate ratio, indicating an enhanced utilization of oxygen by the treated spermatocytes. The simultaneous decrease in the 2,4-dinitrophenol:lactate ratio suggested that spermatocytes were at least partially in an uncoupled oxidative state. The EAA treated cells also showed a large decrease in ATP concentration. The authors concluded the results indicate that EAA interferes with energy metabolism in the pachytene spermatocytes which may partially explain the testicular toxicity. [Description provided by NIOSH]
• Subjects:
  Enzymes Ether Fatty Acids Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Alcohols Aliphatic-compounds Ethers In-vitro-studies In-vivo-studies NIOSH-Grant NIOSH-Publication Reproductive-hazards Reproductive-system-disorders Rodents Testes

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• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  84
• Issue:
  3
• NIOSHTIC Number:
  nn:00163478
• Citation:
  Toxicol Appl Pharmacol 1986 Jul; 84(3):576-583
• Contact Point Address:
  Environmental Health University of Cincinnati 3223 Eden Avenue Cincinnati, Ohio 45267
• CAS Registry Number:
  Ethoxyacetic acid (CAS RN 627-03-2) ; Glycol monoethyl ether (CAS RN 110-80-5)
• Federal Fiscal Year:
  1986
• NORA Priority Area:
  Reproductive System Disorders
• Performing Organization:
  University of Cincinnati, Cincinnati, Ohio
• Peer Reviewed:
  True
• Start Date:
  19810929
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19870831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:84e1ad12187579948128d9a83900ef268857a88b2ed051ce5cc146651372698e27d80768576f62e111957b2a99f9540605f5e8f2bbf499fee15c66d60d35095f
• Download URL:
  https://stacks.cdc.gov/view/cdc/185000/cdc_185000_DS1.pdf
• File Type:
  [PDF - 1.18 MB ]