Two-Dimensional Electrophoretic Protein Profile Analysis Following Exposure of Human Uroepithelial Cells to Occupational Bladder Carcinogens

Details

• Personal Author:
  Kanitz MH ; Savage RE Jr. ; Swaminathan S
• Description:
  Protein biomarkers to occupational carcinogens were investigated using a transformable human uroepithelial cell system, SV-HUC.PC. SV-HUC.PC was treated with N-hydroxy-4,4'-methylene bis (2-chloroaniline) (N-OH-MOCA) or N-hydroxy-4 aminobiphenyl (N-OH-ABP). Two-dimensional gel electrophoresis of cell lysates compared protein changes across treatments. Increasing N-OH-MOCA resulted in a dose-related increase in protein spots altered. Comparing cell profiles treated with either carcinogen revealed alterations in the expression of nine proteins, identified using the TagIdent database. These demonstrated isoelectric point shift (1) or quantity change (8). Our investigation may be useful in identifying biomarkers of effects of exposure to bladder carcinogens. [Description provided by NIOSH]
• Subjects:
  Biomarkers Carcinogens Occupational Health Safety Urogenital Diseases Urogenital System
• Keywords:
  Author Keywords: Uroepithelial Cells Bladder-disorders Bladder Carcinogen Carcinogenesis Occupational-health Protein Biomarkers Proteins Two-dimensional Gel Electrophoresis Urogenital-system-disorders
• ISSN:
  0304-3835
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5 ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  121-131
• Volume:
  205
• Issue:
  2
• NIOSHTIC Number:
  nn:20024438
• Citation:
  Cancer Lett 2004 Mar; 205(2):121-131
• Contact Point Address:
  Division of Applied Research and Technology, Taft Laboratory, National Institute for Occupational Safety and Health, MS/C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
• Email:
  mhk2@cdc.gov
• CAS Registry Number:
  2-Chloroaniline (CAS RN 95-51-2) ; 4-Aminobiphenyl (CAS RN 92-67-1)
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Peer Reviewed:
  True
• Source Full Name:
  Cancer Letters
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2f6db478da289a8c8c9ec5c8290b3af616d7fe1dc20a10ad0e1e32fdc7786f8170d5d8f243988b5e055dd75079657ea1680b32bc04fa530e2931083bdc40afd8
• Download URL:
  https://stacks.cdc.gov/view/cdc/196242/cdc_196242_DS1.pdf
• File Type:
  [PDF - 431.34 KB ]