Influence of industrial poisons and drugs on the activity of microsomal enzymes.

Details

• Personal Author:
  Wisniewska-Knypl JM
• Description:
  The effect of industrial poisons and commonly used drugs on microsomal enzyme activity were studied in Wistar-rats. Rats were given aniline (62533) at 50 milligrams per kilogram (mg/kg) a day for 30 days or 150mg/kg for 3 or 6 days subcutaneously. The rate of microsomal metabolism and the cytochrome-P-450 content were determined after treatment. The roles of the microsomal enzyme inducers phenobarbital (50066) and 3,4-benzpyrene (50328) and the inhibitors SKF-525-A (62680) and cobaltous-chloride (7646799) were studied during aniline metabolism. Azobenzene (55801) was given intraperitoneally to rats at 150mg/kg a day for 3 or 4 days, 25mg/kg for 7 days, or 5mg/kg for 2 or 4 days. Microsomal metabolism of azobenzene and aniline was determined. The effects of common drugs chlordiazepoxide (58253) at 50 to 400mg/kg a day for 5 to 30 days and diazepam (439145) at 100 or 400mg/kg for 5 or 30 days were studied on liver microsomal enzyme activity. Ultrastructural studies were made of liver of rats given chlordiazepoxide. Effects of oxazepam (604751) at 200 or 400mg/kg for 5 or 30 days, phenacetin (62442) at 1 gram/kg for 6 or 14 days, or cyclobarbital (52313) at 150mg/kg for 4, 5, or 6 days were similarly studied. The effects of the drugs on metabolism of aniline and the development of tolerance were studied. Chronic exposure to aniline decreased cytochrome-P- 450 content in the liver and reduced the rate of microsomal metabolism of aniline in-vivo. Inducers stimulated aniline metabolism and inhibitors reduced it. Exposure to azobenzene also reduced microsomal metabolism of azobenzene and of aniline. The tranquilizers chlordiazepoxide and diazepam induced synthesis of microsomal enzymes in the liver. Treatment with chlordiazepoxide caused proliferation of smooth endoplasmic reticulum in parenchymal liver cells. Oxazepam, phenacetin, and cyclobarbital all stimulated microsomal metabolism in the liver. Disappearance of hypothermia and muscle relaxation rate of metabolism increased. [Description provided by NIOSH]
• Subjects:
  Animals Energy Metabolism Occupational Health Safety Toxicology
• Keywords:
  NIOSH-Author; Toxicology; Industrial-chemicals; Hepatic-microsomal-enzymes; Liver-microsomal-enzymes; Quantitative-analysis; Animal-studies; Metabolic-study; Reaction-rates; Quantitative-analysis
• Document Type:
  Text
• Genre:
  Report
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-113
• NIOSHTIC Number:
  nn:00146005
• Citation:
  National Institute of Occupational Safety and Health, U.S. Department of Health, Education and Welfare, Cincinnati, Ohio, Polish-American Agreement No. 05-013-2, 1977 Jun; :1-113
• CAS Registry Number:
  Aniline (CAS RN 62-53-3) ; Benzo[a]pyrene (CAS RN 50-32-8) ; Chlordiazepoxide (CAS RN 58-25-3) ; Cobalt chloride (CoCl2) (CAS RN 7646-79-9) ; Cyclobarbital (CAS RN 52-31-3) ; Diazepam (CAS RN 439-14-5) ; N,N-Dimethyl-4-[2-(3-methylphenyl)diazenyl]benzenamine (CAS RN 55-80-1) ; Oxazepam (CAS RN 604-75-1) ; Phenacetin (CAS RN 62-44-2) ; Phenobarbital (CAS RN 50-06-6) ; Proadifen hydrochloride (CAS RN 62-68-0)
• Federal Fiscal Year:
  1977
• Peer Reviewed:
  False
• Source Full Name:
  National Institute of Occupational Safety and Health, U.S. Department of Health, Education and Welfare, Cincinnati, Ohio, Polish-American Agreement No. 05-013-2, 113 pages, 97 references
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/245674/cdc_245674_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]