Phosphocreatine Restores High-Energy Phosphates in Ischemic Myocardium: Implication for Off-Pump Cardiac Revascularization

Details

• Personal Author:
  Lakhani P ; Murray D ; Murray G ; Prabhakar G ; Vona-Davis L
• Description:
  BACKGROUND: High-energy phosphate metabolism is altered in the ischemic myocardium. We investigated the effects of in vivo administration of phosphocreatine (PCr) in a transient ischemic rat model to emulate off pump myocardial revascularization. STUDY DESIGN: Rats received either PCr (100 mg/kg) or saline intravenously 1 hour before surgery. Regional ischemia was maintained for 12 minutes by ligation of the left anterior descending artery and compared with sham-operated animals. Cardiac tissue was studied for ATP, PCr, and inorganic phosphate (Pi) using P-31-cryo-NMR. Results were compared by ANOVA. RESULTS: Levels of ATP were significantly (p < 0.01) lower in the ischemic hearts compared with controls; Pi and PCr remained unchanged. The PCr/Pi ratio was altered in ischemic hearts, reflecting an increased energy demand. PCr administration significantly (p < 0.01) elevated the content of PCr and ATP in both normal and ischemic hearts. CONCLUSIONS: PCr restores high-energy phosphates and attenuates metabolic stress during periods of myocardial ischemia in the rat. Preconditioning with PCr may serve as a useful adjunct to off-pump coronary revascularization. [Description provided by NIOSH]
• Subjects:
  Animals Cardiovascular System Energy Metabolism Laboratories Musculoskeletal System Occupational Health Phosphoric Acids Safety
• Keywords:
  Adenosines Animal-studies Cardiac-function Cardiovascular-function Cardiovascular-system Energy-metabolism Heart In-vivo-studies Laboratory-animals Metabolic-study Muscle-physiology Myocardium Phosphates Proteins Surgery

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• ISSN:
  1072-7515
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  197
• Issue:
  5
• NIOSHTIC Number:
  nn:20032148
• Citation:
  J Am Coll Surg 2003 Nov; 197(5):786-791
• Contact Point Address:
  West Virginia University, Department of Surgery, P.O. Box 9238, Morgantown, WV 26506-9238
• Federal Fiscal Year:
  2004
• Peer Reviewed:
  True
• Source Full Name:
  Journal of the American College of Surgeons
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:97cef192a826210fd8c7b638ec7a8dd34b2f782834aaed7e1bec44367cc5ad86db003eb7264dddfbb0e269605afafcd57612c122abc517a235d6459516fc7ec8
• Download URL:
  https://stacks.cdc.gov/view/cdc/186195/cdc_186195_DS1.pdf
• File Type:
  [PDF - 100.65 KB ]