In vitro tests for workplace cocarcinogens.

Details

• Personal Author:
  Barfknecht TR ; Hall ME
• Description:
  An in-vitro study of known carcinogens was conducted. Compounds were evaluated for their ability to inhibit metabolic cooperation in an assay system consisting of wild type and mutant (HGPRT-) Chinese-V- 79-hamster cells. Mezerein (34807415), and 12-O- tetradecanoylphorbol-13-acetate (544638) strongly inhibited metabolic cooperation. Sodium-saccharin (128449), sodium- phenobarbital (57307), phorbol (17673255), butylated-hydroxytoluene (128370), butylated-hydroxyanisole (25013165), tert-butyl- hydroperoxide (75912), hydrogen-peroxide (7722841), benzoyl-peroxide (94360), and 2,4-dinitrofluorobenzene (70348) did not inhibit metabolic cooperation. Sodium-cyclamate (139059), tryptophan (153946), and amobarbital (57432) showed ambiguous or weakly positive results. The authors conclude that from comparison of results with those of in-vivo cancer bioassays, the Chinese-V-79- hamster cell assay system correctly identifies tumor promoting ability. The system is most effective in detecting known skin cancer promoters; however, the rate of false negative results is high (50 percent). Further research is recommended to improve the assay system so it can detect weak and non skin tumor promoters and to reduce the number of false negative responses. [Description provided by NIOSH]
• Content Notes:
  in NTRL, no PDF
• Subjects:
  Animals Biochemistry Biological Assay Carcinogens Chemistry Clinical Laboratory Techniques Energy Metabolism Occupational Health Safety
• Keywords:
  NIOSH-Contract; Carcinogenicity; In-vitro-studies; Clinical-chemistry; Animal-studies; Bioassays; Chemical-properties; Biological-effects; Biochemical-tests; Metabolic-study
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Text
• Funding:
  Contract
• Genre:
  Final Contract Report
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-266
• NIOSHTIC Number:
  nn:00134918
• NTIS Accession Number:
  PB84243500
• Citation:
  Cincinnati, OH: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, Contract 200-82-2545, 1984 May; :1-266
• CAS Registry Number:
  2,6-Di-tert-butyl-4-methylphenol (CAS RN 128-37-0) ; Amobarbital (CAS RN 57-43-2) ; Benzoyl peroxide (CAS RN 94-36-0) ; Dinitrofluorobenzene (CAS RN 70-34-8) ; D-Tryptophan (CAS RN 153-94-6) ; Hydrogen peroxide (CAS RN 7722-84-1) ; Mezerein (CAS RN 34807-41-5) ; Myristic acid (CAS RN 544-63-8) ; Phenobarbital sodium (CAS RN 57-30-7) ; Phorbol (CAS RN 17673-25-5) ; Sodium cyclamate (CAS RN 139-05-9) ; Sodium saccharin (CAS RN 128-44-9) ; tert-Butyl hydroperoxide (70% aqueous solution) (CAS RN 75-91-2) ; tert-Butylhydroxyanisole (CAS RN 25013-16-5)
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  False
• Source Full Name:
  National Institute for Occupational Safety and Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/245587/cdc_245587_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]