Inhibition of uranyl nitrate of adenosine triphosphatases derived from animal and human tissues.

Details

• Personal Author:
  Nechay BR ; Saunders JP ; Thompson JD
• Description:
  The effect of uranyl-nitrate (10102064) (UN) on adenosine- triphosphatase (9000833) (ATPase) was studied in dog, cat, hamster, rat, rabbit and human tissue. Brain, heart, kidney, and liver homogenates or microsomal fractions were incubated with UN at 37 degrees C. Fifty percent microsomal ATPase inhibition (I50) required about 0.045 millimolar (mM) UN for dog heart, brain and kidney, while 0.08mM was required in the liver. Human kidney ATPase was twice as resistant to UN as was the dog kidney. The I50 values for dog kidney and brain magnesium ATPase were the same as in the corresponding tissue homogenates. I50 values for the cat, rat, rabbit and hamster kidneys were similar to the dog and human values. Added sodium antagonized the UN inhibition of sodium-potassium ATPase, while added potassium did not. Added ascorbic-acid increased the inhibition of the UN. The authors note that reduced inhibition in sodium-potassium ATPase is observed when the concentrations of ATP alone or ATP and magnesium together are increased indicating that the UN inhibitory action occurs at the ATP binding site. Added albumin or citrate exerts a protective effect, probably due to UN binding to free carboxyl groups. [Description provided by NIOSH]
• Subjects:
  Biochemistry Biological Assay Energy Metabolism Enzymes Laboratories Occupational Health Safety
• Keywords:
  Bioassays Enzyme-activity Laboratory-animals Metabolism NIOSH-Grant NIOSH-Publication
• ISSN:
  0041-008X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 6 ; Texas
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  410-419
• Volume:
  53
• Issue:
  3
• NIOSHTIC Number:
  nn:00112392
• Citation:
  Toxicol Appl Pharmacol 1980 May; 53(3):410-419
• Contact Point Address:
  Pharmacology and Toxicology University of Texas Pharm & Toxicology Department Galveston, Tex 77550
• CAS Registry Number:
  Adenosine triphosphatase (CAS RN 9000-83-3) ; Uranyl nitrate (CAS RN 10102-06-4)
• Federal Fiscal Year:
  1980
• Performing Organization:
  University of Texas Med BR Galveston, Galveston, Texas
• Peer Reviewed:
  True
• Start Date:
  19790401
• Source Full Name:
  Toxicology and Applied Pharmacology
• End Date:
  19850630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ce177a626f8adaf676b1aaf2016d23cf32f208177899a38c9372191861079b6491ce8ece2881a872fed91f8abe321cb340b0e0a65d5623b93bde1bdc6c8ac480
• Download URL:
  https://stacks.cdc.gov/view/cdc/184405/cdc_184405_DS1.pdf
• File Type:
  [PDF - 1.27 MB ]