In vitro tests for workplace cocarcinogens.

Details

• Personal Author:
  Gulati DK ; Russell S ; Sabharwal PS
• Description:
  In-vitro assays of workplace cocarcinogens were conducted. Compounds selected by NIOSH were evaluated for their ability to block metabolic cooperation between wild type and mutant Chinese-V-79- hamster cells. The concentrations of the compounds ranged from 0.01 to 25000 micrograms per milliliter. The inhibition of metabolic cooperation was evaluated by determining the number of surviving mutant cells. Mezerein (34807415), 12-o-tetradecanoylphorbol-13- acetate (544638), amobarbital (57432), butylated-hydroxytoluene (128370), phenobarbital (50066), butylated-hydroxyanisole (25013165), tryptophan (153946), and catechol (120809) inhibited metabolic cooperation between the wild type and mutant cells. Sodium-cyclamate (139059), 2,4-dinitrofluorobenzene (70348), benzoyl- peroxide (94360), phorbol (17673255), n-dodecane (112403), tert- butyl-hydroperoxide (75912), hydrogen-peroxide (7722841), and ethyl- phenylpropiolate (2216946) had no significant effect on metabolic cooperation. The overall response from the remaining compounds sodium-saccharin (128449), 1-phenyldodecane (123013), pyrogallol (87661), phenol (108952), and sucrose (57501) was either ambiguous or weakly positive. Tert-butyl-hydroperoxide and n-dodecane enhanced metabolic cooperation. The observed response of sucrose may represent changes in the osmolarity of the culture medium. Of the compounds tested, 15 have been accurately assessed for their ability to block metabolic cooperation. [Description provided by NIOSH]
• Content Notes:
  in NTRL, no PDF
• Subjects:
  Animals Biochemistry Biological Assay Carcinogens Chemistry Clinical Laboratory Techniques Energy Metabolism Occupational Health Safety
• Keywords:
  NIOSH-Contract; Carcinogenicity; In-vitro-studies; Clinical-chemistry; Animal-studies; Bioassays; Chemical-properties; Biological-effects; Biochemical-tests; Metabolic-study
• Publisher:
  National Institute for Occupational Safety and Health
• Document Type:
  Text
• Funding:
  Contract
• Genre:
  Final Contract Report
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-199
• NIOSHTIC Number:
  nn:00134779
• Citation:
  Cincinnati, OH: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, Contract 200-82-2544, 1984 May; :1-199
• CAS Registry Number:
  2,6-Di-tert-butyl-4-methylphenol (CAS RN 128-37-0) ; 2-Propynoic acid, 3-phenyl-, ethyl ester (CAS RN 2216-94-6) ; Amobarbital (CAS RN 57-43-2) ; Benzoyl peroxide (CAS RN 94-36-0) ; Catechol (CAS RN 120-80-9) ; Dinitrofluorobenzene (CAS RN 70-34-8) ; Dodecane (CAS RN 112-40-3) ; Dodecylbenzene (CAS RN 123-01-3) ; D-Tryptophan (CAS RN 153-94-6) ; Hydrogen peroxide (CAS RN 7722-84-1) ; Mezerein (CAS RN 34807-41-5) ; Myristic acid (CAS RN 544-63-8) ; Phenobarbital (CAS RN 50-06-6) ; Phenol (CAS RN 108-95-2) ; Phorbol (CAS RN 17673-25-5) ; Pyrogallol (CAS RN 87-66-1) ; Sodium cyclamate (CAS RN 139-05-9) ; Sodium saccharin (CAS RN 128-44-9) ; Sucrose (CAS RN 57-50-1) ; tert-Butyl hydroperoxide (70% aqueous solution) (CAS RN 75-91-2) ; tert-Butylhydroxyanisole (CAS RN 25013-16-5)
• Federal Fiscal Year:
  1984
• Peer Reviewed:
  False
• Source Full Name:
  National Institute for Occupational Safety and Health
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/245575/cdc_245575_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]