Details:

Alternative Title:
Emerg Infect Dis
Publisher's site:
http://dx.doi.org/10.3201/eid2008.131399
Personal Author:
den Bakker, Henk C. ; Allard, Marc W. ; Bopp, Dianna ; Brown, Eric W. ; Fontana, John ; ... More +
den Bakker, Henk C. ; Allard, Marc W. ; Bopp, Dianna ; Brown, Eric W. ; Fontana, John ; Iqbal, Zamin ; Kinney, Aristea ; Limberger, Ronald ; Musser, Kimberlee A. ; Shudt, Matthew ; Strain, Errol ; Wiedmann, Martin ; Wolfgang, William J. Less -
Description:
For Salmonella enterica serovar Enteritidis, 85% of isolates can be classified into 5 pulsed-field gel electrophoresis (PFGE) types. However, PFGE has limited discriminatory power for outbreak detection. Although whole-genome sequencing has been found to improve discrimination of outbreak clusters, whether this procedure can be used in real-time in a public health laboratory is not known. Therefore, we conducted a retrospective and prospective analysis. The retrospective study investigated isolates from 1 confirmed outbreak. Additional cases could be attributed to the outbreak strain on the basis of whole-genome data. The prospective study included 58 isolates obtained in 2012, including isolates from 1 epidemiologically defined outbreak. Whole-genome sequencing identified additional isolates that could be attributed to the outbreak, but which differed from the outbreak-associated PFGE type. Additional putative outbreak clusters were detected in the retrospective and prospective analyses. This study demonstrates the practicality of implementing this approach for outbreak surveillance in a state public health laboratory.
Subject:
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Bacteria
High-throughput Nucleotide Sequencing
Infectious Disease Outbreaks
Public Health Laboratory Surveillance
Pulsed-field Gel Electrophoresis
Research
Salmonella Enterica Serovar Enteritidis
Whole-genome Sequencing

Source:
Emerg Infect Dis. 20(8):1306-1314.
Document Type:
Journal Article
Collection(s):
Emerging Infectious Diseases
Main Document Checksum:
[+]
urn:sha256:60c2019423c56d4d8b3b293ea5045a72ab303582c1c93d0656c84d23dba271d1
File Type:

Supporting Files

• 	license.txt	txt
  	13-1399-F1.gif	gif
  	13-1399-F1.jpg	jpeg
  	13-1399-F2.gif	gif
  	13-1399-F2.jpg	jpeg
  	13-1399-Techapp-s1.pdf	pdf
  	13-1399.nxml	txt

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