Effects of platelet activating factor on membrane potential and respiratory burst activity of human granulocytes.

Details

• Personal Author:
  Castranova, Vincent ; Van Dyke K
• Description:
  The effects of platelet activating factor (PAF) on membrane potential and respiratory burst activity of human granulocytes were studied in-vitro. Granulocytes prepared from blood drawn from healthy volunteers were incubated with 0 to 10(-4) molar PAF in the presence or absence of extracellular sodium ion (Na+) or calcium-45 (Ca-45) tagged calcium-chloride. The effects on transmembrane potential and hydrogen-peroxide release were determined by fluorescence techniques. Oxygen consumption was measured. Superoxide anion release was determined by a spectrophotometric procedure. Chemiluminescence of the cells was measured by liquid scintillation counting. Uptake of Ca-45 was measured. PAF caused a rapid transient depolarization of the granulocyte membranes in the presence of extracellular Na+. In the absence of Na+, PAF had no effect on membrane potential. Uptake of Ca-45 was not affected by PAF or the presence or absence of extracellular calcium-chloride. PAF treated cells generated chemiluminescence only when Na+ was present in the medium. PAF induced a moderate release of hydrogen- peroxide and a weak release of superoxide anion. Oxygen consumption was not affected. The authors conclude that PAF causes a Na+ dependent activation of human granulocytes. This response may be involved in the inflammatory process. [Description provided by NIOSH]
• Subjects:
  Blood Laboratories Occupational Health Safety
• Keywords:
  In-vitro-studies; Blood-cells; Humans; Bioactivation; Physiological-chemistry; Laboratory-techniques; Platelets; Cell-function; Bioelectric-effects; NIOSH-Author
• ISBN:
  9780849343322
• Publisher:
  CRC Press
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  Florida ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  158-166
• NIOSHTIC Number:
  nn:00176484
• Citation:
  Cellular chemiluminescence. Van Dyke K, Castranova V, eds. Boca Raton, FL: CRC Press, 1987 Jul; :158-166
• Editor(s):
  Dyke K; Castranova V
• Federal Fiscal Year:
  1987
• Peer Reviewed:
  False
• Source Full Name:
  Cellular chemiluminescence
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
• Download URL:
  https://stacks.cdc.gov/view/cdc/242426/cdc_242426_DS1.pdf
• File Type:
  [PDF - 168.02 KB ]