Meeting Guidelines for Cholinesterase Monitoring by Clinical Laboratories in California

Details

• Personal Author:
  Arrieta DE ; Henderson JD ; O'Malley, Michael A. ; Wilson BW
• Description:
  California (CA) is the only state with a long standing formal monitoring program for mixers, loaders and applicators of pesticides. When we found commercial clinical kits were not optimal for assaying blood cholinesterases (ChEs), CA regulations were revised to specify use of optimal Ellman ChE assay conditions. Alternate methods were allowed if a correlation (r 2) of 0.9 was achieved in a comparison with the specified method. We were enlisted to work with the clinical laboratories. Only 2 of 7 participating laboratories achieved an acceptable correlation for red blood cell acetylcholinesterase (AChE) and 4 of 5 laboratories for plasma ChE. When the CA Department of Pesticide Regulation (DPR) reiterated the need to meet this requirement we used bovine ghost RBC AChE as a standard to work with several of the clinical laboratories. Only 3 of 10 participating laboratories had acceptable correlations. Some said their instruments lacked the sensitivity to assay the bovine AChE samples. Next, we provided all interested laboratories with human blood and plasma samples to perform a comparison study outlined in the regulation (Section 6728f). Fourteen laboratories participated; 9 met the criterion for whole blood, 14 for plasma and 6 for RBC AChE. Based on such data, DPR notified the CA Agricultural Commissioners on July 8, 2003 that 9 clinical laboratories were approved for ChE testing. We continue to work with laboratories interested in being on the approved list. [Description provided by NIOSH]
• Subjects:
  Agriculture Blood Cholinesterase Inhibitors Farmers Insecticides Laboratories Occupational Health Safety
• Keywords:
  Agricultural-chemicals Agricultural-workers Blood-cells Cholinesterase-inhibitors Clinical-tests Laboratory-testing Monitoring-systems Pesticides Pesticides-and-agricultural-chemicals Red-blood-cells
• ISSN:
  1096-6080
• Publisher:
  Society of Toxicology
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; Maryland ; OSHA Region 3 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  172-173
• Volume:
  78
• NIOSHTIC Number:
  nn:20025080
• Citation:
  Toxicologist 2004 Mar; 78(S-1):172-173
• Federal Fiscal Year:
  2004
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  False
• Start Date:
  19900930
• Source Full Name:
  The Toxicologist. Society of Toxicology 43nd Annual Meeting and ToxExpo, March 21-25, 2004, Baltimore, Maryland
• Supplement:
  S-1
• End Date:
  20020929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6c26d644e16e3391f8603602d28bb0efdb3f59a7c865c178be0dc4ca3877c9e05758f7f45500d74b97cd6569fdfc501cf2a46787d2ed559d84d5a19f000230cf
• Download URL:
  https://stacks.cdc.gov/view/cdc/196582/cdc_196582_DS1.pdf
• File Type:
  [PDF - 66.32 KB ]